摘要
目的:探究紫薯花青素提取工艺参数,测定其稳定性和含量,并对其进行抗氧化、抗紫外活性的初步研究。方法:采用单因素试验和正交试验优化紫薯花青素的提取工艺,测定紫薯花青素在不同条件下的稳定性。通过还原力、自由基清除实验和AAPH斑马鱼氧化应激模型评价紫薯花青素的抗氧化活性;通过斑马鱼UVB损伤模型测定紫薯花青素的抗紫外损伤作用。结果:在料液比为1∶15(g/mL)、温度为60℃、溶剂为95%乙醇、超声提取时间为30 min时,紫薯花青素的含量为29.27%;紫薯花青素溶液在温度60℃、pH 4~5、避光条件下稳定性较高。0.03~0.10 mg/mL紫薯花青素溶液具有显著的还原力以及清除羟基和DPPH自由基的能力;0.05 mg/mL紫薯花青素溶液提高了AAPH模型组斑马鱼体内SOD、CAT活力;0.10 mg/mL紫薯花青素溶液修复了UVB照射引起的斑马鱼尾鳍皱缩。结论:紫薯花青素的最佳提取工艺为95%乙醇、料液比1∶15(g/mL)、温度60℃、超声提取30 min,紫薯花青素溶液具有一定的抗氧化以及修复UVB损伤的作用。
Objective:To explore the extraction process parameters of anthocyanins from purple potato,determine the stability and content,and conduct preliminary studies on the antioxidant and anti-ultraviolet activities.Methods:Single factor test and orthogonal test were used to optimize the extraction process of anthocyanins from purple potato.The stability of purple potato anthocyanins under different conditions was further measured.The antioxidant activity of purple potato anthocyanins was evaluated by reducing power,free radical scavenging experiment and AAPH zebrafish oxidative stress model.The anti-ultraviolet damage effect of anthocyanins from purple potato was determined by zebrafish UVB damage model.Results:The content of anthocyanins from purple potato was 29.27% when the solid-liquid ratio was 1∶15(g/mL),the temperature was 60℃,the solvent was 95% ethanol,and the ultrasonic extraction time was 30 min.The purple potato anthocyanin solution exhibited high stability at a temperature of 60℃,a pH range of 4-5,and under light-shielded conditions.0.03-0.10 mg/mL purple potato anthocyanin solution had significant reducing power and the ability to scavenge hydroxyl and DPPH free radicals.0.05 mg/mL purple potato anthocyanin solution increased the activities of SOD and CAT in the AAPH zebrafish model group.0.10 mg/mL purple potato anthocyanin solution repaired the tail fin shrinkage of zebrafish caused by UVB.Conclusion:The optimal extraction process of anthocyanins from purple potato was 95% ethanol,solid-liquid ratio 1∶15(g/mL),temperature 60℃,ultrasonic extraction for 30 min.The anthocyanin solution had certain antioxidant activities and could repair UVB damage.
作者
孙姗姗
熊蕾
杨洋
束成杰
王文强
陈斌
高巍
聂韡
Sun Shanshan;Xiong Lei;Yang Yang;Shu Chengjie;Wang Wenqiang;Chen Bin;Gao Wei;Nie Wei(Jiangsu Institute of Product Quality Supervision and Inspection,Nanjing 210000,China;Nanjing Institute for Comprehensive Utilization of Wild Plants,China Co-ops,Nanjing 211111,China;School of Traditional Chinese Pharmacy,China Pharmaceutical University,Nanjing 210009,China)
出处
《中国野生植物资源》
CSCD
2024年第12期10-18,共9页
Chinese Wild Plant Resources
基金
江苏省市场监督管理局科技计划项目(KJ2023013)。
关键词
紫薯花青素
提取工艺
稳定性
抗氧化
抗紫外
斑马鱼
Anthocyanins from purple potato
Extraction process
Stability
Antixidant
Anti-ultraviolet
Zebrafish
作者简介
孙姗姗(1985-),女,博士,副研究员,研究方向为食品质量研究。E-mail:shanshans112@163.com;通讯作者:聂韡(1982-),女,博士,研究员,研究方向为植物资源开发及其在化妆品方面的应用。E-mail:47884537@qq.com。
