摘要
目的:研究黄芪甲苷对酵母粉联合腺嘌呤灌胃给药建立的大鼠痛风性肾病(Gouty Nephropathy,GN)模型的炎症反应水平、氧化应激水平和肾功能的影响。方法:从50只健康雄性SD大鼠中随机取8只大鼠作为对照组,其余42只大鼠为造模组。通过联合酵母粉和腺嘌呤灌胃诱导大鼠GN模型,造模第8天从造模组中随机选取2只大鼠,处死后取肾脏,进行苏木精-伊红(HE)染色法染色,观察肾脏组织,判断是否造模成功。造模成功后,将造模组40只大鼠随机分为5组:模型组、别嘌醇组、黄芪甲苷低剂量组、黄芪甲苷中剂量组、黄芪甲苷高剂量组,每组8只。各组灌胃给药干预4周。使用HE染色法观察肾脏组织切片,以了解肾脏的微观结构和病理变化。同时,通过酶联免疫吸附试验(Enzyme-linked Immunosorbent Method,ELISA)检测大鼠血清中肿瘤坏死因子-α(Tumor Necrosis Factor-α,TNF-α)、白细胞介素-1β(Interleukin-1β,IL-1β)、尿酸(Uric Acid,UA)、尿素氮(Blood Urea Nitrogen,BUN)、肌酐(Creatinine,Cr)、黄嘌呤氧化酶(Xanthine Oxidase,XOD)、肾损伤分子-1(Kidney Injury Molecule-1,KIM-1)、活性氧(Reactive Oxygen Species,ROS)、还原性谷胱甘肽(Reduced Glutathione,GSH)水平。结果:与模型组相比较,别嘌醇组和黄芪甲苷低剂量组、黄芪甲苷中剂量组、黄芪甲苷高剂量组大鼠血TNF-α、IL-1β水平明显降低,差异具有统计学意义(P<0.01);血UA、XOD、KIM-1水平显著降低,GSH水平升高,差异具有统计学意义(P<0.01);血BUN和ROS水平均显著降低,差异具有显著的统计学意义(P<0.01,P<0.05);此外,黄芪甲苷中剂量组和黄芪甲苷高剂量组大鼠的血Cr水平也显著降低,差异同样具有高度的统计学意义(P<0.01)。结论:黄芪甲苷对GN大鼠具有较好的肾保护作用,可能与降低大鼠炎症反应、氧化应激反应及血尿酸水平有关。
Objective:To study the effects of astragaloside on the level of inflammatory response,oxidative stress and renal function in a rat model of gouty nephropathy(GN)established by gavage administration of yeast powder combined with adenine.Methods:There were 50 healthy male SD rats,eight rats were randomly taken as the control group,and the remaining 42 rats were as the modeling group.The rat GN model was induced by yeast powder and adenine gavage,and on the 8th day of modeling,two rats in the modeling group were randomly selected to be executed,the kidneys were extracted,and the kidney tissues were stained with HE staining to observe the kidney tissues to determine whether the modeling was successful or not.After successful modeling,40 rats in the modeling group were randomly divided into five groups:the model group,the allopurinol group,the astragaloside low dosage group,the astragaloside medium dosage group,and the astragaloside high dosage group,with 8 rats in each group.Each group was given the drug by gavage for 4 weeks.HE staining was used to observe the kidney tissue sections to understand the microstructure and pathological changes of the kidney.The kidney tissue sections were stained with HE staining,and tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),uric acid(UA),blood urea nitrogen(BUN),creatinine(Cr),xanthine oxidase(XOD),kidney injury molecule-1(KIM-1),reactive oxygen species(ROS)and reduced glutathione(GSH)in the serum of the rats were detected by ELISA.Results:Compared with the model group,the levels of blood TNF-αand IL-1βin the allopurinol group,the astragaloside low dosage group,the astragaloside medium dosage group,and the astragaloside high dosage group were significantly lower,with statistically significant differences(P<0.01).The levels of blood UA,XOD and KIM-1 were significantly lower,and the level of GSH was higher,with statistically significant differences(P<0.01).The levels of blood BUN and ROS were significantly lower,with statistically significant differences(P<0.01,P<0.05).Blood Cr in rats in the astragaloside medium dosage group,and the astragaloside high dosage group was significantly reduced,and the difference was statistically significant(P<0.01).Conclusion:Astragaloside has a better nephroprotective effect on rats with gouty nephropathy,which may be related to the reduction of inflammatory response,oxidative stress,and blood UA level in rats.
出处
《中医临床研究》
2024年第35期83-87,共5页
Clinical Journal Of Chinese Medicine
基金
基于NF-κB/NLRP3信号通路探究黄芪甲苷对大鼠痛风性肾病的作用机制(202311834027)。
关键词
黄芪甲苷
痛风性肾病
炎症因子
氧化应激
肾功能
Astragaloside
Gouty nephropathy
Inflammatory factor
Oxidative stress
Renal function
