摘要
目的制备一种共同负载姜黄素和IR1061染料的新型纳米脂质体,并研究其对肝癌细胞的抗肿瘤/光热联合治疗效果。方法采用薄膜水化法制备脂质体IR1061@姜黄素-Lips。对其粒径大小、稳定性、表面Zeta电位、多分散系数(PDI)、包封率、光热转换能力及光热稳定性进行考察;细胞水平上,利用激光扫描共聚焦显微镜探讨肝癌细胞对IR1061@姜黄素-Lips的摄取行为;通过细胞毒性实验,评估IR1061@姜黄素-Lips对肝癌细胞的抗肿瘤+光热联合治疗效果。结果IR1061@姜黄素-Lips的平均粒径(176.77±10.17)nm,分布均匀,稳定性良好,姜黄素药物的包封率为(64.79±2.62)%;带负电位,Zeta电位为(-37.17±0.41)mV;PDI为(0.242±0.018);在近红外808 nm激光照射下具有良好的光热升温效果,光热稳定性好,计算得光热转换效率高达47.45%;细胞实验结果表明脂质体的形式增加了肝癌细胞对药物的摄取,姜黄素与IR1061的联合对肝癌细胞有显著的抗肿瘤/光热协同抑制作用。结论成功制备了新型纳米脂质体IR1061@姜黄素-Lips,证明了其对肝癌细胞有显著的抗肿瘤/光热协同抑制作用,为肝癌患者在治疗方式的选择上提供了一种新的可能。
Objective To prepare a novel nanoliposome loaded with curcumin and IR1061 dye,and to study its anti-tumor/photothermal combined therapy effect on hepatocellular carcinoma cells.Methods Liposomes IR1061@curcumin-Lips were prepared by thin film hydration.The particle size,stability,surface Zeta potential,polydispersity index(PDI),encapsulation efficiency,photothermal conversion ability,and photothermal stability were investigated.At the cellular level,laser scanning confocal microscopy was used to investigate the uptake behavior of IR1061@curcumin-Lips by hepatocellular carcinoma cells.The effect of IR1061@curcumin-Lips combined with anti-tumor and photothermal on hepatocellular carcinoma cells was evaluated by cytotoxicity test.Results The average particle size of IR1061@curcumin-Lips was(176.77±10.17)nm,which was uniform distribution and good stability.The encapsulation efficiency of curcumin was(64.79±2.62)%.The Zeta potential was(-37.17±0.41)mV.PDI was(0.242±0.018);it had a good photothermal heating effect and good photothermal stability under the near-infrared 808 nm laser irradiation,and the photothermal conversion efficiency was as high as 47.45%.The results of cell experiments showed that the liposome form of curcumin increased the drug uptake of hepatoma cells,and the combination of curcumin and IR1061 had a significant anti-tumor/photothermal syner-gistic inhibition effect on hepatocellular carcinoma cells.Conclution A novel nano-liposome IR1061@curcumin-Lips has been successfully prepared,and it has a significant anti-tumor/photothermal synergistic inhibition effect on liver cancer cells,which is expected to provide a new idea for the treatment of liver cancer.
作者
潘虹
向兴华
赵倩茹
李佩
陈青山
朱朝庚
PAN Hong;XIANG Xinghua;ZHAO Qianru;LI Pei;CHEN Qingshan;ZHU Chaogeng(The First Clinical College of Traditional Chinese Medicine,Hunan University of Chinese Medicine,Hunan Province,Changsha410000,China;Department of Hepatobiliary,Pancreatic,and Hernia Surgery,the First Affiliated Hospital of Hunan University of Chinese Medicine,Hunan Province,Changsha410000,China)
出处
《中国医药导报》
2024年第34期25-30,共6页
China Medical Herald
基金
湖南省自然科学基金面上基金项目(2023JJ 30479)
湖南中医药管理局科研计划项目(D2022082)
湖南省长沙市杰出创新青年培养计划项目(kq2306029)。
关键词
脂质体
姜黄素
IR1061
抗肿瘤/光热治疗
联合治疗
肝细胞癌
Liposomes
Curcumin
IR1061
Anti-tumor/photothermal therapy
Combination therapy
Hepatocellular carcinoma
作者简介
潘虹(1998.11-),女,湖南中医药大学第一中医临床学院2022级中西医结合专业在读硕士研究生,研究方向:肝胆胰系统疾病;通讯作者:陈青山(1989.11-),男,博士,研究方向:纳米中药在肝胆胰疾病中的应用;通讯作者:朱朝庚(1971.2-),男,博士,主任医师,硕士生导师,研究方向:肝胆胰疾病的基础及临床研究。
