摘要
【目的】研究硼(B)和硅(Si)对紫花苜蓿(MedicagosativaL.)铝毒害的解毒作用。【方法】采取温室水培试验,筛选硼和硅最佳缓解浓度,以最佳浓度设置7个处理(CK、Al、B、Si、Al+B、Al+Si、Al+B+Si),测定紫花苜蓿生长指标、根尖柠檬酸代谢和抗氧化指标。【结果】无铝条件下,硼和硅促进紫花苜蓿生长;在铝胁迫下,紫花苜蓿根中铝含量显著增加,根长、根生物量、茎叶簇高、茎叶生物量(簇)和叶绿素含量显著降低(P<0.05),幼苗的生长明显受到铝抑制;添加硼和硅后,根中铝积累显著降低(P<0.05),且硼和硅共同作用显示出更好的效果。此外,硼和硅虽然提高了根尖柠檬酸合酶活性,但根尖柠檬酸含量和分泌量降低,因此B和Si缓解铝毒并不是通过柠檬酸分泌的途径来实现。硼和硅还能增加抗氧化酶活性,减少MDA和H_(2)O_(2)的含量,从而缓解铝毒。【结论】B和Si对紫花苜蓿生长有促进作用,对铝毒有缓解作用,且两者协同效果更佳。
【Objective】To study the detoxifying effects of boron(B)and silicon(Si)on aluminum poison-ing in alfalfa(Medicago sativa L.).【Method】The greenhouse hydroponics trial was carried out.Firstly,the optimal concentrations of B and Si were screened,and 7 treatments(CK,Al,B,Si,Al+B,Al+Si,Al+B+Si)were set at the optimal concentrations to determine the growth indicators,root tip citric acid metabolism,and antioxidant indicators of alfalfa.【Result】The addition of boron and silicon could promote the growth of alfalfa without aluminum stress.Under aluminum treatment,the aluminum content in alfalfa root was signifi-cantly increased,while root length,root biomass,stem and leaf cluster height,stem and leaf biomass(clus-ters)and chlorophyll content were significantly decreased,indicating that the growth of alfalfa seedlings was significantly inhibited by aluminum.After the addition of boron and silicon,the accumulation of aluminum in the root was significantly reduced,and the combined effect of boron and silicon showed a better effect.In ad-dition,although boron and silicon increased the activity of apical citric acid synthase,the content and secre-tion of citric acid in the apical tip decreased,so that the alleviation of aluminum toxicity by boron and silicon was not achieved by citric acid secretion.Boron and silicon can also increase the activity of antioxidant en-zymes and reduce the content of MDA and H_(2)O_(2),thus alleviating aluminum toxicity.【Conclusion】Boron and silicon can promote alfalfa growth and alleviate the aluminum toxicity,and the synergistic effect of the two is better.
作者
李冰洋
苟少校
郭海海
郭炎峰
魏运民
蒋林峰
玉永雄
LI Bingyang;GOU Shaoxiao;GUO Haihai;GUO Yanfeng;WEI Yunmin;JIANG Linfeng;YU Yongxiong(College of Animal Science and Technology,Southwest University,Chongqing 404100,China;Chongqing Ani-mal Husbandry Extension Station,Chongqing 404100,China)
出处
《甘肃农业大学学报》
CSCD
北大核心
2024年第6期227-235,共9页
Journal of Gansu Agricultural University
基金
重庆市技术创新与应用发展专项(NO.cstc2021jscx-msxmX0009)。
关键词
硅
铝毒害
解毒作用
硼
紫花苜蓿
silicon
aluminum toxicity
detoxification
boron
Medicago sativa L.
作者简介
第一作者:李冰洋,硕士研究生。E-mail:2476054094@qq.com;通信作者:玉永雄,教授,研究方向为牧草遗传资源、牧草育种与草地生产。E-mail:yuyongxiong8@126.com。
