摘要
[目的]本文旨在探明水分胁迫下不同氮素形态对水稻根系通气组织形成的影响及该过程中的调控因子。[方法]以‘汕优63’(杂交籼稻)和‘扬稻6号’(常规籼稻)为试验材料,采用室内营养液培养,通过供应不同氮素形态以及100 g·L^(-1)PEG6000模拟水分胁迫的方法,分析了不同氮素形态和不同水分条件对水稻根系解剖结构及生理生化特性的影响。[结果]模拟水分胁迫条件下供硝态氮营养水稻根系通气组织面积显著大于供铵态氮营养水稻,且其形成时间早于后者;与供铵态氮营养水稻相比,模拟水分胁迫条件下供硝态氮营养水稻根系脯氨酸含量低,丙二醛含量高,抗胁迫能力差;与供铵态氮营养水稻相比,模拟水分胁迫条件下供硝态氮营养水稻根系呼吸速率显著增加,活性氧清除酶(超氧化物歧化酶、过氧化氢酶和过氧化物酶)活性下降,超氧阴离子大量累积。[结论]抗氧化能力下降和活性氧调控系统失衡引发的活性氧大量积累是导致水分胁迫条件下供硝态氮营养水稻根系通气组织显著增大的原因。
[Objectives] This paper was aimed to study the effects of different nitrogen forms and water stress on aerenchyma formation of rice root and the regulatory factor in the process.[Methods] A hydroponic experiment with simulated water stress induced by polyethylene glycol（PEG6000） was conducted in greenhouse to study the effects of different nitrogen（N） forms on aerenchyma formation of rice root.Two rice cultivars（‘ Shanyou 63’ hybrid indica and‘ Yangdao 6’ indica,China）were grown under water stress condition （100 g＆#183; L-1 polyethylene glycol 6000）,supplying of different N forms （nitrate and ammonium）.We investigated the physiological and biochemical characteristics,and anatomical structure of rice roots under different treatments.[Results] Plants supplied with nitrate nutrition significantly triggered root aerenchyma formation than those supplied with ammonium nutrition under water stress condition.Plants supplied with nitrate nutrition had lower proline content,higher MDA content and lipid peroxidation level than those supplied with ammonium nutrition under water stress condition.Water stress significantly enhanced root respiration,and decreased the root reactive oxygen species （ROS） scavenging enzymes activities of rice plants supplied with nitrate nutrition accompanied by higher superoxide anion content,when compared with rice plants supplied with ammonium nutrition.[Conclusions] Consequently,the decreased antioxidant capability and accumulation of ROS owing to ROS scavenging enzymes system imbalance result in greater aerenchyma formation in root of rice plants supplied with nitrate nutrition under water stress conditions.
出处
《南京农业大学学报》
CAS
CSCD
北大核心
2017年第2期273-280,共8页
Journal of Nanjing Agricultural University
基金
国家自然科学基金项目(30871587)
关键词
氮素形态
水分胁迫
通气组织
活性氧清除酶
超氧阴离子
nitrogen form
water stress
aerenchyma
reactive oxygen species scavenging enzymes
superoxide anion
作者简介
陈钰佩，硕士研究生。
通信作者：郭世伟，教授，主要研究方向为植物营养生理学、作物施肥理论与实践和植物营养与病害E-mail：sguo@njau．edu．cn。
