摘要
本研究利用白木香无菌种苗顶芽为外植体,研究不同激素配比对芽诱导和生根的影响,获得一套白木香快速繁殖技术。结果表明:赤霉素能提高白木香种子的发芽率,最适合种子萌发的培养基为A3:MS+GA31.0 mg/L;最适合白木香顶芽诱导丛生芽的培养基为B3:MS+6-BA 1.0 mg/L+NAA 0.1+KT 0.5 mg/L;最适合白木香组培苗生根的培养基为C3:1/2MS+IBA 1.0 mg/L+NAA 0.5 mg/L。本研究为实现今后白木香的工厂化生产奠定了一定基础。
Using the terminal buds of Aquilaria sinensis sterile seedlings as explants, the effects of different hormones to shoots induction and rooting were studied, to obtain a set of rapid propagation technology of Aquilaria sinensis. Results showed that GA3 could improve seeds germination rate, and the most suitable medium for seed germination was A3: MS +GA31.0 mg/L, and medium B3: MS +6-BA 1.0 mg/L +NAA 0.1 +KT 0.5 mg/L could induce clustered shoots and multiplication best, the most suitable medium for rooting was C3: 1/2MS+IBA 1.0 mg/L+NAA 0.5 mg/L. This study in order to achieve the next white costus factory production laid a certain foundation.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2015年第6期1296-1299,共4页
Genomics and Applied Biology
基金
海南省中药现代化专项"白木香组织培养技术与标准化育苗技术的研究"(ZY201409)资助
关键词
白木香
组织培养
芽诱导
生根
Aquilaria sinensis,Tissue culture,Shoot induction,Rooting
