摘要
以盾叶薯蓣茎段为外植体,在生根培养基中添加不同浓度的Ce^3+,观察其对盾叶薯蓣组培苗生根和抗氧化酶系统及活性氧代谢水平的影响。结果表明,0.5-20mg·L^-1的Ce^3+对盾叶薯蓣组培苗生根有明显的促进作用,可显著提高外植体的生根率、单株发根数、根的鲜重等,5mg·L^-1的Ce^3+显示出最强的促进效应,而高浓度的Ce^3+呈抑制效应。培养基中添加合适浓度的Ce^3+对组培苗叶片中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)等有显著的调节作用,有效抑制·O2^-的生成速率和丙二醛(MDA)的积累,显示出Ce^3+对组培苗促进生根的效应与调节活性氧代谢水平的一致性。
Different concentrations of Ce (NO3)3 were supplemented to the culture medium (1/2 MS + 0.5μmol· L^-1 IBA) to study its effects on the rooting, antioxidative enzyme systems and the level of AOS metabolism of the tissue culture plantlet from D. zingberensis. The results show that appropriate concentrations of Ce^3+ (0.5 - 20 mg·L^-1 ) obviously increase the rooting rate, the root number of a single plantlet and roots' fresh weights, and 5 mg·L^-1 Ce^3+ have the most remarkable effect, while higher concentrations of Ce^3+ (20 - 30 mg· L^-1) has the opposite effect. In addition, Ce^3+ in suitable concentrations can regulate the activities of SOD and CAT, and control · O2^- production and MDA accumulation at the same time.
出处
《中国稀土学报》
CAS
CSCD
北大核心
2006年第3期380-384,共5页
Journal of the Chinese Society of Rare Earths
基金
科技部项目(2003DEB6J074)
国家发改委项目(GJX01100626)
江苏省科技厅项目(BG2001045)
关键词
铈
盾叶薯蓣
生根效应
活性氧代谢
稀土
cerium
D. zingiberensis
rooting
AOS metabolism
rare earths
作者简介
金春雁(1980-),女,江苏太仓人,硕士研究生;研究方向:植物生理生化
通讯联系人(E-mail:jasmonic589@sohu.com)