摘要
不同产地的天冬总氨基酸含量存在差异。文章以水提法为基础,以L-天冬酰胺为对照,采用分光光度法,用2%茚三酮和磷酸二氢钾-氢氧化钠溶液来显色,并在568 nm下测定吸光度,对11个不同产地的天冬进行总氨基酸含量测定,进而计算天冬的总氨基酸含量。结果表明,天冬的总氨基酸含量较高,且不同产地的含量差异较大;L-天冬酰胺在4~14μg/m L质量浓度范围内与吸光度呈现良好的线性关系(R^(2)=0.9992),回归方程为A=0.0727c+0.003,平均回收率101.429%,SRSD=3.9%。该方法可被应用于测定天冬的总氨基酸含量。
The paper aimed to compare the content of total amino acids in Asparagus cochinchinensis from different producing areas.Amino acids were extracted by water extraction method,L-asparagine was used as reference substance,spectrophotometry was used,2%ninhydrin and potassium dihydrogen phosphate-sodium hydroxide buffer were used for color development,and the absorbance was measured at 568 nm.The content of total amino acids in Asparagus cochinchinensis from 11 different habitats was determined,and the content of total amino acids in Asparagus cochinchinensis was calculated.The content of total amino acid in Asparagus cochinchinensis was high,and the contents in different producing areas were different.The concentration of L-asparagine in the range of 4-14μg/mL showed a good linear relationship with absorbance(R^(2)=0.9992),and the regression equation was A=0.0727c+0.003.The average recovery was 101.429%,S_(RSD)=3.9%.This method can be used for the content determination of total amino acids in Asparagus cochinchinensis.
作者
孟繁盛
卢星烨
龚帅
许恒
汪大巍
MENG Fansheng;LU Xingye;GONG Shuai;XU Heng;WANG Dawei(Wuhan Huaxia Institute of Technology,Wuhan 430223,China)
出处
《工业微生物》
CAS
2024年第6期139-141,共3页
Industrial Microbiology
关键词
天冬
总氨基酸
产地
含量测定
可见分光光度法
Asparagus cochinchinensis
total amino acids
producing areas
content determination
visible spectrophotometry
作者简介
孟繁盛(2002-),男,汉族,河北张家口人,本科生在读,研究方向:中药活性成分提取与分析;通信作者:汪大巍,男,汉族,硕士,讲师,研究方向:中药活性成分提取与分析。E-mail:fakan0000@126.com。
