摘要
为探讨羧基化单壁碳纳米管(SWCNTs-COOH)的生物效应,本研究选用“品育8012”小麦为实验材料,采用水培的方式研究不同浓度SWCNTs-COOH(0、5、10、30、50、70和90 mg·L^(-1))对低钾(K+,0.01 mmol·L^(-1))胁迫下小麦幼苗生长发育的影响。结果表明:5~50 mg·L^(-1)的SWCNTs-COOH对小麦幼苗的生长指标(株高、根系、叶面积、鲜质量、干质量)、光合作用指标(细胞色素含量、叶绿素荧光参数)、抗氧化酶(SOD、POD)活性均存在一定的促进作用,其中50 mg·L^(-1)浓度时促进效果最明显;当SWCNTs-COOH的浓度达到70 mg·L^(-1)及以上时小麦幼苗的生长指标、光合作用指标、SOD活性、POD活性逐渐降低,丙二醛(MDA)含量明显上升,即高浓度的SWCNTs-COOH加重了低钾胁迫下小麦幼苗的毒理学效应。综上,SWCNTs-COOH对低钾胁迫下小麦幼苗生长发育的影响存在明显的浓度效应,低浓度的SWCNTs-COOH可以缓解低钾胁迫对小麦幼苗造成的部分损伤,高浓度的SWCNTs-COOH会对小麦幼苗产生一定的生理毒性。
To explore the biological effects of carboxylated single-walled carbon nanotubes(SWCNTs-COOH),this work selected“Pinyu 8012”wheat as the experimental material and used hydroponics cultivation method to investigate the effects of various concentrations of SWCNTs-COOH(0,5,10,30,50,70,and 90 mg·L^(-1))on the growth and development of wheat seedlings under low potassium stress(K+,0.01 mmol·L^(-1)).With the most notable impact being observed at a concentration of 50 mg·L^(-1),the results revealed that SWCNTs-COOH concentrations ranging from 5 to 50 mg·L^(-1) positively affected growth parameters(plant height,root system,leaf area,fresh weight,dry weight),photosynthetic indicators(cytochrome content,chlorophyll fluorescence parameters),and antioxidant enzyme(SOD,POD)activities in wheat seedlings.However,at doses of 70 mg·L^(-1) or higher,growth in dicators,photosynthetic parameters,SOD activity,and POD activity in wheat seedlings dropped while the malondial-dehyde(MDA)content rose dramatically.This implies that high quantities of SWCNTs-COOH exacerbate the toxicological effects of low potassium stress on wheat.In summary,there is a significant concentration effect of S WCNTs-COOH on the growth and development of wheat seedlings under low potassium stress.Low concentrations of SWCNTs-COOH can help to reduce some of the low potassium stress-induced damage in wheat seedlings,while high concentrations of SWCNTs-COOH can cause certain physiological toxicity to wheat seedlings.
作者
何艳华
张全喜
郭东罡
HE Yanhua;ZHANG Quanxi;GUO Donggang(College of Environment and Resource,Shanxi Laboratory for Yellow River,Shanxi University,Taiyuan 030006,China;Shanxi University of Electronic Science and Technology,Linfen 041000,China;Shanxi Normal University,Taiyuan 030000,China)
出处
《生态毒理学报》
CAS
CSCD
北大核心
2024年第6期140-151,共12页
Asian Journal of Ecotoxicology
基金
山西省基础研究计划青年科学研究项目(202203021212390)。
关键词
羧基化单壁碳纳米管
小麦
生理毒性
钾胁迫
生长发育
carboxylated single-walled carbon nanotubes
wheat
physiological toxicity
potassium stress
growth and development
作者简介
第一作者:何艳华(1986-),女,博士,副教授,硕士生导师,研究方向为生态毒理学,E-mail:sxheyanhua@126.com;通信作者:张全喜(1980-),男,博士,教授,博士生导师,主要研究方向为生态环境毒理学。E-mail:qxzhang@sxu.edu.cn;共同通信作者:郭东罡(1980-),男,博士,教授,博士生导师,主要研究方向为生态恢复与治理。E-mail:gdghjkx@126.com。
