摘要
研究采用平板计数法,评估了两个团体标准(T/YNBX 024,简称云南团标;T/CSWSL 022,简称北京团标)中的检测培养基及其pH对饲用凝结芽孢杆菌制剂的菌数检测影响。结果显示:两个团体标准中的特异培养基均可用于饲用凝结芽孢杆菌的菌数检测。采用云南团标配制的检测培养基,培养基pH 4.9~5.5范围均可用于准确检测凝结芽孢杆菌的总菌数。然而,采用北京团标中的检测培养基,需将培养基pH调整为5.5。以上研究结果旨在为规范饲用凝结芽孢杆菌制剂的菌数检测方法提供科学依据。
In this experiment,culture medium and its pH in two existing promulgated standards T/YNBX 024(Yunnan group standard)and T/CSWSL 022(Beijing group standard)was evaluated to the aerobic count detection of probiotics Bacillus coagulans additive by plate count method.The results showed that two culture media in above promulgated standards could be available to detect the bacteria count of probiotics Bacillus coagulans additive.Moreover,for the aerobic counting accuracy,the range of culture media pH 4.9-5.5 for Yunnan group standard was observed,but culture media pH 5.5 for Beijing group standard should be adjusted and regulated.These findings provide a scientific basis for standardizing to the aerobic count of probiotics Bacillus coagulans additive by plate count method.
作者
王雪艳
王乙茹
苗华彪
陈德近
何雪
杨睿
安清聪
程志斌
WANG Xueyan;WANG Yiru;MIAO Huabiao;CHEN Dejin;HE Xue;YANG Rui;AN Qingcong;CHENG Zhibin(Faculty of Animal Science and Technology,Yunnan Agricultural University,Yunnan Kunming 650201,China;Dazhou Vocational and Technical College,Sichuan Dazhou 635001,China;School of Life Science Yunnan Normal University,Yunnan Kunming 650222,China;Qactive Bio-Engineering(Kunming)Co.,Ltd.,Yunnan Kunming 650506,China)
出处
《饲料工业》
CAS
北大核心
2024年第24期91-95,共5页
Feed Industry
基金
国家自然科学基金项目[31860664]
云南省农业联合面上项目[2017FG001-067]。
关键词
饲用凝结芽孢杆菌
菌数检测
培养基
平板计数法
团体标准
probiotics Bacillus coagulans additive
aerobic counting
culture medium
plate count method
group standard
作者简介
王雪艳,硕士,研究方向为动物营养与饲料科学;通讯作者:程志斌,副教授,硕士生导师。
