摘要
[目的]参考ISO19036:2019对饮用水中大肠菌群的测量不确定度进行评估。[方法]通过设计试验方案,分别评估技术不确定度(u_(tech))、基质不确定度(u_(matrix))和分布不确定度(u_(distrib)),并综合3个分量的数值计算合成不确定度(uc(y))和扩展不确定度(U)。[结果]对GB 4789.3—2016第二法进行测量不确定度评估后,得到的技术不确定度为0.1202 log(CFU/mL),基质不确定度为0.1 log(CFU/mL),分布不确定度分别为0.0655,0.0261 log(CFU/mL),当结果为440 CFU/mL时,扩展不确定度为0.34 log(CFU/mL),表示为(2.64±0.34)log(CFU/mL)。[结论]ISO19036:2019对食品中微生物定量检测方法进行测量不确定度评估相较目前国内标准更科学、更便捷。
[Objective]This study aimed to estimate the measurement uncertainty of coliforms in drinking water according to ISO19036:2019.[Methods]By designing protocols,the technical uncertainty(u_(tech)),matrix uncertainty(u_(matrix))and distribution uncertainty(u_(distrib))were estimated respectively,and the combined uncertainty(uc(y))and extended uncertainty(U)were calculated by combining the values of the three components.[Results]After estimating the measurement uncertainty of the second method of GB 4789.3—2016,the technical uncertainty was 0.1202 log(CFU/mL),the matrix uncertainty was 0.1 log(CFU/mL),and the distribution uncertainty was 0.0655 log(CFU/mL)and 0.0261 log(CFU/mL).When the result was 440 CFU/mL,the extended uncertainty was 0.34 log(CFU/mL),expressed as(2.64±0.34)log(CFU/mL).[Conclusion]According to ISO19036:2019,the estimation of measurement uncertainty for quantitative determinations of microorganisms in food is more scientific and convenient compared to current domestic standards.
作者
钟文涛
卢琳
李政
周培华
洪振柏
周丛
ZHONG Wentao;LU Lin;LI Zheng;ZHOU Peihua;HONG Zhenbai;ZHOU Cong(Hunan Provincial Institute of Product and Commodity Goods Quality Inspection,Changsha,Hunan 410007,China;Hunan Provincial Key Laboratory of Food Safety Monitoring and Early Warning,Changsha,Hunan 410007,China)
出处
《食品与机械》
CSCD
北大核心
2024年第10期68-72,共5页
Food and Machinery
基金
湖南省市场监督管理局科技计划项目(编号:2024KJJH05)
湖南省自然科学基金—部门联合基金项目(编号:S2024JJBMLH0442)。
关键词
测量不确定度
技术不确定度
基质不确定度
分布不确定度
measurement uncertainty
technical uncertainty
matrix uncertainty
distributional uncertainty
作者简介
通信作者:周丛(1978-),男,湖南省产商品质量检验研究院正高级工程师,硕士。E-mail:3489785@qq.com。
