摘要
紫外分光光度法因其可操作性高、仪器价格便宜,被广泛应用于分析方法中,本文以产地为湖南的芡实为研究对象,采用分光光度法,对其中钙、铜两种微量元素的含量进行测定。测量前进行了显色剂稳定时间、最大吸收波长、显色剂用量三个因素的探究,为验证测定方法的适用性,本研究采用了加标回收率的策略。测定钙元素的显色剂为酸性铬兰K,确定了最大吸收波长为470 nm,显色剂稳定时间选择12 min,使用2.5 mL,绘制出钙元素标准曲线,并得到了拟合方程A=0.0140c+0.0124(R^(2)=0.9992),加标回收率实验的平均值为95.4%,测得钙含量为29.6573μg/g。测定铜元素,采用了DDTC(二乙基二硫代氨基甲酸钠)作为显色剂,确定最大吸收波长为449 nm,显色剂稳定时间选择10 min,使用3 mL,绘制出铜元素标准曲线,得到拟合方程为A=0.1588c+0.0307(R^(2)=0.9992),加标回收率实验的平均值为97.1%,测得铜含量为9.6953μg/g。
Ultraviolet spectrophotometry is widely used in analytical methods because of its high operability and cheap instrument.In this paper,Euryale ferox from Hunan Province was the research object,and the content of calcium and copper two trace elements was determined by spectrophotometry.Before the measurement,three factors of color developer stability time,maximum absorption wavelength and color developer dosage were investigated.In order to verify the applicability of the measurement method,the strategy of adding standard recovery rate was adopted in this study.The color developer of calcium was determined to be acid chromic blue K,the maximum absorption wavelength was determined to be 470 nm,the stability time of color developer was selected for 12 min,and 2.5 mL was used to draw the standard curve of calcium,and the fitting equation A=0.0140c+0.0124(R^(2)=0.9992)was obtained.The average recovery rate was 95.4%,the content of calcium was 29.6573μg/g.For the determination of copper,DDTC(sodium diethyl dithiocarbamate)was used as the color developing agent,the maximum absorption wavelength was determined to be 449 nm,the stability time of the color developing agent was selected for 10 min,and the standard curve of copper was drawn using 3 mL.The fitting equation was obtained as A=0.1588c+0.0307(R^(2)=0.9992).The average recovery rate of the experiment was 97.1%,the copper content was 9.6953μg/g.
作者
何激忠
滕谦
刘芯言
李波
唐珊
陈梓鑫
彭莘馨
奉馨怡
HE Jizhong;TENG Qian;LIU Xinyan;LI Bo;TANG Shan;CHEN Zixin;PENG Xinxin;FENG Xinyi(Aba Teachers University,Aba 623002,China)
出处
《山东化工》
CAS
2024年第21期139-142,共4页
Shandong Chemical Industry
基金
国家级大学生创新创业项目(S202210646006)
阿坝师范学院实验教学质量提升项目(ASS20210308)
阿坝师范学院质量工程项目(20220701051)。
关键词
芡实
分光光度法
钙元素
铜元素
测定
Euryale ferox
spectrophotometry
calcium
copper
determination
作者简介
何激忠,阿坝师范学院资源与环境学院学生;通信作者:李波,助教,主要研究方向为无机化学。
