摘要
目的观察血必净注射液预处理对造影剂急性肾损伤(CI-AKI)大鼠肾小管损伤的影响.方法选择24只SD大鼠,按随机数字表法分为正常组、模型组、对照组、治疗组,每组6只.采用碘海醇制备CI-AKI动物模型,正常组不进行任何处理.治疗组大鼠于制模前15h开始经尾静脉注射血必净注射液,至制模24h结束,注射剂量10 mL/kg,每6h1次;对照组于相应时间点给予相同剂量的生理盐水.制模24h后收集各组大鼠尿液、血液,测定尿素氮(BUN)、血肌酐(SCr)、尿N-乙酰基-β-D-葡萄糖苷酶(uNAG)水平,然后将大鼠处死取肾脏组织,行苏木素-伊红(HE)染色,光镜下观察肾脏组织病理学变化.结果模型组BUN、SCr和uNAG均较正常组明显升高[BUN(μmol/L):37.29±6.18比6.37±1.19,SCr(mmol/L):30.43±4.44比14.90±1.61,uNAG(U/L):47.77±4.71比11.32±3.62,均P<0.01];治疗组BUN、SCr和uNAG水平均较模型组明显下降[BUN(μmol/L):9.45±3.04比37.29±6.18,SCr(mmol/L):19.83±2.16比30.43±4.44,uNAG(U/L):21.70±6.21比47.77±4.71,均P<0.05];但治疗组BUN、uNAG仍明显高于正常组[BUN(μmol/L):9.45±3.04比6.37±1.19,uNAG(U/L):21.70±6.21比11.32±3.62,P<0.05或P<0.01],SCr与正常组比较差异无统计学意义(μmol/L:19.83±2.16比14.90±1.61,P>0.05).光镜下可见:模型组大鼠肾脏皮质及皮髓交界处肾小管上皮细胞明显空泡化,伴细胞脱落、坏死,肾小管扩张,肾小球无明显病变;对照组及治疗组与模型组相比损伤程度减轻.模型组肾小管损伤程度高于正常组;对照组和治疗组肾小管损伤程度均较模型组降低,治疗组与对照组肾小管损伤程度比较差异无统计学意义.结论血必净注射液可能通过减轻肾小管损伤,发挥保护CI-AKI大鼠肾功能的作用.
Objective To investigate the effects of pretreatment with Xuebijing injection on renal tubular injury in rats with contrast-induced acute kidney injury(CI-AKI).Methods Twenty-four Sprague-Dawley(SD)rats were selected and divided into normal group,model group,control group,and treatment group according to the random number table method,with 6 rats in each group.The animal model of CI-AKI was prepared by adopting iohexol,and the normal group was not subjected to any treatment.The rats in the treatment group were injected with Xuebijing injection via the tail vein 15 hours before modeling until 24 hours after modeling.The injection volume was 10 mL/kg for every 6 hours.The control group was injected with normal saline at the same time point.After 24 hours of modeling,the urine of rats in each group was collected to determine the levels of blood urea nitrogen(BUN)and urine N-acetyl-β-D-gluco-aminidase(uNAG),and the blood was collected to determine the levels of serum creatinine(SCr).Then the rats were killed and the kidney tissues were extracted,and then stained with hematoxylin-eosin(HE),and the pathological changes of the kidney tissues were observed under the light microscope.Results BUN,SCr and uNAG were significantly higher in the model group than those in the normal group[BUN(μmol/L):37.29±6.18 vs.6.37±1.19,SCr(mmol/L):30.43±4.44 vs.14.90±1.61,uNAG(U/L):47.77±4.71 vs.11.32±3.62,all P<0.01];BUN,SCr and uNAG levels were obviously decreased in the treatment group compared to the model group[BUN(μmol/L):9.45±3.04 vs.37.29±6.18,SCr(mmol/L):19.83±2.16 vs.30.43±4.44,uNAG(U/L):21.70±6.21 vs.47.77±4.71,all P<0.05],however,BUN and uNAG in the treatment group were still significantly higher than those in the normal group[BUN(μmol/L):9.45±3.04 vs.6.37±1.19,uNAG(U/L):21.70±6.21 vs.11.32±3.62,P<0.05 or P<0.01];SCr in the treatment group was not statistically significant compared to the normal group(μmol/L:19.83±2.16 vs.14.90±1.61,P>0.05).Under the light microscope,the renal tubular epithelial cells at the junction of cortex and dermatomedulla in the kidneys of the model group were obviously vacuolated,accompanied by cell detachment and necrosis,and the tubules were dilated,with no obvious lesions in the glomeruli.The degree of damage in the control group and the treatment group was reduced compared with that in the model group.The degree of renal tubular damage in the model group was higher than that in the normal group;while the degree of renal tubular damage in the control group was significantly lower than that in the model group;and the degree of renal tubular damage in the treatment group was lower than that in the model group.There was no statistically significant difference in the degree of renal tubular damage between the treatment group and the control group.Conclusion Xuebijing injection may exert a protective effect on renal function in rats with CI-AKI by attenuating renal tubular injury.
作者
武子霞
张曼钰
李朝园
赵富浩
董淑慧
鲁胜
武雅文
杨定位
Wu Zixia;Zhang Manyu;Li Chaoyuan;Zhao Fuhao;Dong Shuhui;Lu Sheng;Wu Yawen;Yang Dingwei(Department of Nephrology,Tianjin Hospital,Tianjin University,Tianjin 30021l,China;Tianjin University,Tianji 300072,China;The Second Hospital of Shanxi Medical University,Taiyuan 030001,Shanxi,China)
出处
《中国中西医结合急救杂志》
CAS
CSCD
2024年第4期447-450,共4页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
天津市医药卫生中医中西医结合科研课题(2021200)。
关键词
造影剂急性肾损伤
血必净注射液
肾小管损伤
Contrast-induced acute kidney injury
Xuebijing injection
Renal tubular injury
作者简介
通信作者:杨定位,Email:dxyang0072003@163.com。
