摘要
目的:探讨针刺治疗帕金森病便秘的作用机制。方法:检索近10年针灸治疗帕金森病便秘的文献,筛选出治疗前后明显改变的活性成分,通过PubChem和Swiss Target Prediction数据库获得其关联靶点。确定活性成分和疾病靶点的交集,构建PPI网络,通过微生信平台对治疗的关键靶点分别进行GO功能和KEGG信号通路富集分析,得到蛋白的分子功能、细胞组分、参与的生物过程和通路。结果:通过预测分析得到171个与针灸治疗帕金森病便秘相关的基因靶点,KEGG富集分析通路10条。结论:针刺治疗帕金森病便秘(PDC)可能通过DRD2、GRM5、SLC6A3、MAOB及HTR1A等核心靶点调节DA、α-Syn、NE和5-HT水平,主要通路有钙离子信号通路、G蛋白偶联受体信号通路和神经活性配体-受体相互作用等。
Objective:To investigate the mechanism of acupuncture in treatment of Parkinson's disease-related constipation(PDC).Methods:The literature on acupuncture treating PDC over the past 10 years was reviewed.Active ingredients that exhibited significant changes before and after the treatment were identified,and their related targets were retrieved from PubChem and Swiss Target Prediction database.The intersection of active ingredients and disease targets was determined,the PPI network was constructed,and the molecular functions,cell components,biological processes and pathways of proteins involved were obtained by enrichment analysis of GO function and KEGG signaling pathways of key therapeutic targets through the Bioinformatics platform.Results:Predictive analysis identified 171 gene targets associated with acupuncture treatment for PDC,and revealed 10 KEGG enrichment analysis pathways.Conclusion:Acupuncture may regulate DA,α-Syn,NE and 5-HT levels through core targets of DRD2,GRM5,SLC6A3,MAOB and HTR1A in treatment of PDC,the main signaling pathway calcium signaling pathway,G protein-coupled receptor signaling pathway,and neuroactive ligand-receptor interaction.
作者
张哲
马伊笛
秦霈瑶
李帛珈
李冀
ZHANG Zhe;MA Yidi;QIN Peiyao;LI Bojia;LI Ji(Heilongjiang University of Chinese Medicine,Harbin 150040,China)
出处
《针灸临床杂志》
2024年第11期69-74,共6页
Journal of Clinical Acupuncture and Moxibustion
基金
中医药传承与创新百千万人才工程(岐黄工程)国家中医药领军人才支持计划,编号:国中医药人教函[2018]284号
国家中医药管理局李冀名老中医药专家传承工作室项目(2014年)。
关键词
帕金森
便秘
针刺疗法
生物信息学
信号通路
分子机制
Parkinson s disease
Constipation
Acupuncture therapy
Bioinformatics
Signaling pathway
Molecular mechanism
作者简介
张哲(1997-),男,2022级中医内科学专业硕士研究生,研究方向:中医临床方药学研究;通讯作者:李冀(1960-),男,博士研究生导师,教授,从事方剂配伍规律研究。
