摘要
该研究采用传统培养分离法、纤维素降解酶活力及玉米秸秆降解率的测定,从玉米秸秆中分离、筛选具有纤维素降解能力的菌株,并通过形态学观察及分子生物学技术对其进行菌种鉴定。综合考虑菌株门类、纤维素降解酶活性和玉米秸秆降解效果筛选优良菌株,考察各菌株间的拮抗性,并构建不同复合菌系(编号为S1~S8)。通过比较不同复合菌系的纤维素降解酶活力,最终构建高效秸秆降解复合菌系。结果表明,筛选、鉴定得到7株高效降解秸秆的优良菌株,分别为巨大芽孢杆菌(Priestia megaterium)S381-8、短短芽孢杆菌(Brevibacillus brevis)S381-7、解淀粉芽孢杆菌(Bacillus amyloliquefaciens)S381-3、贝莱斯芽孢杆菌(Bacillus velezensis)S381-4、杆菌状链霉菌(Streptomyces bacillaris)S381-2、里氏木霉(Trichoderma reesei)S876-2、产紫青霉(Penicillium purpurogenum)S876-6,各菌株间无拮抗性。以菌株S876-6、S876-2、S381-3、S381-2为中心菌株,协同菌株S381-7、S381-4、S381-8(各菌株菌悬液体积比1∶1)混合构建的复合菌系S8纤维素降解酶活力(25.58 U/mL)最高,并且经其处理后的秸秆表面物理结构更为疏松多孔,表明该复合菌系具有良好的秸秆降解潜力。
In this study,the strains with cellulose degradation ability were isolated and screened from corn straw by traditional culture and separation method,detecting cellulose-degrading enzyme activities and corn straw degradation rates,and identified by morphological observation and molecular biology techniques.Considering the strain phylum,cellulose-degrading enzyme activity and corn straw degradation effect,the superior strains were screened,the antagonism between strains was investigated,and different composite microbial system(number as S1-S8)were constructed.By comparing the cellulose-degrading enzyme activities of different composite microbial system,the high-efficiency straw degradation composite microbial system was finally constructed.The results showed that 7 superior strains with high efficiency of straw degradation were screened and identified,which were Priestia megaterium S381-8,Brevibacillus brevis S381-7,Bacillus amyloliquefaciens S381-3,Bacillus velezensis S381-4,Streptomyces bacillaris S381-2,Trichoderma reesei S876-2,Penicillium purpurogenum S876-6.There was no antagonism among the strains.The composite microbial system S8 was constructed with strains S876-6,S876-2,S381-3,and S381-2 as the central strains,and cooperative strains S381-7,S381-4,and S381-8(the suspension volume ratio of each strain 1∶1),the cellulose-degrading enzyme activity(25.58 U/ml)was the highest,and the physical structure of straw surface after treatment with the composite microbial system S8 was more porous,indicating that the composite microbial system had good straw degradation potential.
作者
陈海霞
潘大伟
全艳玲
孙昊然
赵立男
张雨虹
CHEN Haixia;PAN Dawei;QUAN Yanling;SUN Haoran;ZHAO Linan;ZHANG Yuhong(School of Chemical Engineering,University of Science and Technology Liaoning,Anshan 114051,China;Ansteel Group Mining Co.,Ltd.,Anshan 114001,China)
出处
《中国酿造》
CAS
北大核心
2024年第11期70-76,共7页
China Brewing
基金
国家重点研发计划(2018YFF0213406)。
关键词
秸秆降解菌
纤维素酶活性
筛选
鉴定
复合菌系
straw-degrading strain
cellulase activity
screening
identification
complex microbial system
作者简介
陈海霞(1998-),女,硕士研究生,研究方向为微生物菌剂的开发与应用;通讯作者:全艳玲(1967-),女,副教授,硕士,研究方向为生物工程。
