摘要
目的:对狭叶茴香Illicium lanceolatum A.C.Smith果实进行化学成分研究。方法:采用硅胶柱、Sephadex LH-20葡聚糖凝胶、RP-C18柱、MCI gel柱以及半制备高效液相等色谱技术进行分离纯化,根据波谱数据结合文献数据鉴定化合物结构,并进行抗肿瘤活性筛选。结果:从狭叶茴香果实中分离得到11个化合物,分别鉴定为:(1R)-3-methoxy-3,6-dimethyl-2,3-dihydrobenzofuran(1)、原儿茶酸甲酯(2)、原儿茶酸(3)、水杨酸(4)、间羟基苯甲酸甲酯(5)、没食子酸甲酯(6)、邻羟基肉桂酸(7)、伪大八角素(8)、10β-hydroxy-cyclopseudoanisatin(9)、2,3-didehydro-5-O-methyl-11-epiillifunone E(10)、莽草酸(11)。结论:其中,化合物1为新化合物,化合物2、4~10为首次从该植物中分离得到。化合物1和10显示出一定的抗肿瘤活性,IC_(50)分别为(27.22±0.06)μmol/L、(21.13±0.44)μmol/L。
Objective:To investigate the chemical constituents from the fruits of Illicium lanceolatum.Methods:Silica gel,Sephadex LH-20,RP-C18,MCI gel,semi-preparative HPLC and other chromatography techniques were used for the isolation and purification.The structures of the compounds were identified based on spectral data and literature data,and the antitumor activity was screened.Results:Eleven compounds were isolated from the fruits of Illicium lanceolatum and identified as(1R)-3-methoxy-3,6-dimethyl-2,3-dihydrobenzofuran(1),methyl 3,4-dihydroxybenzoate(2),protocatechuic acid(3),salicylic acid(4),methyl 3-hydroxybenzoate(5),methyl gallate(6),o-hydroxycinnamic acid(7),pseudoanisatin(8),10β-hydroxy-cyclopseudoanisatin(9),2,3-didehydro-5-O-methyl-11-epiillifunone E(10),shikimic acid(11),respectively.Conclusion:Among them,compound 1 is a new compound,compounds 2,4 to 10 are isolated from Illicium lanceolatum for the first time.Compounds 1 and 10 show strong antitumor activity with the IC_(50)of(27.22±0.06)μmol/L,(21.13±0.44)μmol/L,respectively.
作者
邹奇缘
许旭东
马国需
袁经权
ZOU Qi-yuan;XU Xu-dong;MA Guo-xu;YUAN Jing-quan(Scientific Experimental Center of Guangxi University of Chinese Medicine,Nanning 530200,China;Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100193,China)
出处
《中药材》
CAS
北大核心
2024年第3期610-614,共5页
Journal of Chinese Medicinal Materials
基金
国家自然科学基金项目(82060700)。
关键词
狭叶茴香
化学成分
Illicium lanceolatum A.C.Smith
Chemical constituents
作者简介
邹奇缘(1999-),女,在读硕士研究生,专业方向:中药化学成分研究与应用,E-mail:2465833158@qq.com;通讯作者:袁经权,E-mail:yjqgx@163.com。
