摘要
目的采用现代分析技术对破伤风类毒素(TT)的结构进行表征。方法采用圆二色谱法(CD)对TT的二级结构进行考察,差示扫描量热法(DSC)对TT的变性温度进行考察,并对不同批次样品的测定结果进行比较。结果9批次TT的α-螺旋比例为25.4%~26.2%,反向平行为12.3%~14.9%,平行为6.4%~7.0%,β-转角为21.7%~24.0%,无规卷曲为31.8%~33.9%,熔解温度为89.19℃~90.47℃,结果重复性、批间一致性均良好。结论圆二色谱法和差示扫描量热法可用于破伤风类毒素的结构表征,为该类产品在生产过程中的稳定性和一致性提供技术支持。
Objective To characterize the structure of tetanus toxoid(TT)using modern analytical techniques.Methods Circular dichroism(CD)was used to investigate the secondary structure of TT,differential scanning calorimetry(DSC)was used to investigate the denaturation temperature of TT,then the determination results of different batches of samples were compared.Results 9 batches of TTα-helix ratio is 25.4%~26.2%.The anti-parallel is 12.3%~14.9%.The parallel is 6.4%~7.0%.Theβ-turn is 21.7%~24.0%,the random coil is 31.8%~33.9%,and the melting temperature is 89.19℃~90.47℃.The repeatability and inter batch consistency of the results are good.Conclusion Circular dichroism and differential scanning calorimetry can be used for structural characterization of tetanus toxoids,providing technical support for the stability and consistency of such products in the production process.
作者
杨海艳
刘贤雪
杜剑晖
罗玲
詹益雄
车腾
郭长福
瞿明霞
YANG Hai-yan;LIU Xian-xue;DU Jian-hui(Wuhan Institute of Biological Products Co.,Ltd.,Wuhan 430207,China)
出处
《中国处方药》
2024年第10期53-55,共3页
Journal of China Prescription Drug
关键词
破伤风类毒素
圆二色谱
差示扫描量热法
二级结构
变性温度
Tetanus toxoid
Circular dichroism
Differential scanning calorimetry
Secondary structure
Denaturation temperature
作者简介
通信作者:瞿明霞,Email:122523774@qq.com。
