摘要
目的研究在临床血脂生化检验中应用分级检验方法的检验效果。方法选择2022年1月—2023年12月在青岛市黄岛区第三人民医院接受生化检验的90例患者作为研究对象,采集所有患者的两份血液标本,分别应用常规拉网式检验和分级检验方法检测血脂指标,采用酶法测定总胆固醇(TC)、三酰甘油(TG),采用直接法-表面活性剂测定低密度脂蛋白胆固醇(LDL-C),采用直接法-选择抑制法测定高密度脂蛋白胆固醇(HDL-C),采用免疫透射比浊法对载脂蛋白A1(ApoA1)、载脂蛋白B(ApoB)进行检测。比较两种检验方法所得检验结果以及阳性检出率的差异,记录检验时间和检测费用,计算漏检率。结果采用分级检验所得LDL-C、ApoA1、ApoB的测定值以及阳性率均显著高于常规拉网式检验〔LDL-C(mmol/L):4.50±0.62比2.90±0.51;ApoA1(g/L):1.88±0.35比1.32±0.22;ApoB(g/L):1.34±0.25比0.86±0.13;LDL-C阳性率:96.67%比70.00%;ApoA1阳性率:24.44%比3.33%;ApoA1阳性率:42.22%比0%;均P<0.05〕。分级检验的检验时间显著短于常规拉网式检验(min:18.11±1.57比23.75±3.69,P<0.05),检测费用显著少于常规拉网式检验(元:30.44±2.80比46.60±3.71,P<0.05),漏检率显著低于常规拉网式检验(4.44%比20.00%,P<0.05)。结论与常规拉网式检验方法比较,血脂生化检验分级检验的检测时间更短,且具有更高的准确率。
Objective To study the effectiveness of applying grading testing method in clinical blood lipid biochemical testing.Methods The 90 patients who underwent biochemical tests at the Third People's Hospital of Huangdao District,Qingdao City from January 2022 to December 2023 were selected as research subjects.Two samples of blood were collected from all patients,and routine screening test and grading testing method were used to detect blood lipid indicators,respectively.Total cholesterol(TC)and triacylglycerol(TG)were measured using enzymatic methods,low-density lipoprotein cholesterol(LDL-C)was measured using direct method surfactant method,high-density lipoprotein cholesterol(HDL-C)was measured using direct method selective inhibition method,and apolipoprotein A1(ApoA1)and apolipoprotein B(ApoB)were detected using immunoturbidimetry method.The test results and positive detectable rates obtained from two testing methods were compared,the test time and cost were recorded,and the missed detectable rates were calculated.Results The measured values and positive rates of LDL-C,ApoA1 and ApoB obtained through grading testing method were significantly higher than those obtained by routine screening test[LDL-C(mmol/L):4.50±0.62 vs.2.90±0.51;ApoA1(g/L):1.88±0.35 vs.1.32±0.22;ApoB(g/L):1.34±0.25 vs.0.86±0.13;positive rate of LDL-C:96.67%vs.70.00%;positive rate of ApoA1:24.44%vs.3.33%;positive rate of ApoA1:42.22%vs.0%;all P<0.05].The testing time of grading testing method was significantly shorter than that of routine screening test(minutes:18.11±1.57 vs.23.75±3.69,P<0.05),the testing cost was significantly lower than that of routine screening test(Yuan:30.44±2.80 vs.46.60±3.71,P<0.05),and the missed detectable rate was significantly lower than that of routine screening test(4.44%vs.20.00%,P<0.05).Conclusion Compared to routine screening test,grading testing method for blood lipid biochemical test has shorter detection time and higher accuracy.
作者
唐秀欣
Tang Xiuxin(Department of Clinical Laboratory,the Third People's Hospital of Huangdao District,Qingdao City,Qingdao 266409,Shandong,China)
出处
《实用检验医师杂志》
2024年第3期274-277,共4页
Chinese Journal of Clinical Pathologist
关键词
血脂
生化检验
分级检验
Blood lipid
Biochemical test
Grading testing method
作者简介
通信作者:唐秀欣,Email:txx1309@163.com。
