摘要
以结球甘蓝(Brassica oleracea L.var.capitata L.)种子为试材,采用盆栽污染模拟试验,研究不同程度Cd胁迫(0、25、50、100、200、400 mg·L^(-1))对其种子萌发特性、幼苗芽和根伸长、生物量、叶绿素含量、叶片丙二醛含量、叶片相对电导率、叶片渗透调节物质含量(可溶性糖和可溶性蛋白质)、根系活力的影响,以期为十字花科芸薹属植物繁育以及土壤Cd污染所致植物损害程度鉴定提供参考依据。结果表明:1)高浓度(>100 mg·L^(-1))的Cd胁迫显著降低了结球甘蓝种子的发芽率、发芽势、发芽指数和活力指数,而低浓度(<100 mg·L^(-1))的Cd胁迫对发芽势和发芽率的影响不显著,但导致了发芽指数和活力指数的下降。2)不同浓度的Cd胁迫均抑制了结球甘蓝幼苗芽和根的伸长、根系活力和叶绿素含量的积累,而生物量、可溶性糖和可溶性蛋白质含量仅在高于100 mg·L^(-1)的Cd胁迫下才表现出抑制作用。此外,高于100 mg·L^(-1)的Cd胁迫还导致了结球甘蓝幼苗叶片相对电导率的显著升高。但不同浓度的Cd胁迫均未对丙二醛含量和根冠比产生显著影响。综上可知,结球甘蓝种子萌发和幼苗生长对低于100 mg·L^(-1)的Cd胁迫具有一定的耐受性,而Cd浓度高于100 mg·L^(-1)时会对结球甘蓝种子萌发和幼苗生长产生较大的抑制作用。
Taking seeds of Brassica oleracea L.var.capitata L.as the test material under different levels of Cd stress(0,25,50,100,200 mg·L^(-1) and 400 mg·L^(-1)).The effects of different Cd concentrations on seed germination characteristics,seedling shoot and root elongation,biomass,chlorophyll content,leaf malondialdehyde content,leaf relative conductivity,leaf osmoregulatory substances content(soluble sugars and soluble proteins),and root vigor of Brassica oleracea L.var.capitata L.were investigated in a potting contamination simulation experiment,in order to provide reference for the breeding of Brassica oleracea L.var.capitata L.and identification of plant damage due to soil Cd contamination.The results showed that,1)high concentration(>100 mg·L^(-1))of Cd stress significantly reduced the germination rate,germination potential,germination index and vigor index of Brassica oleracea L.var.capitata L.seeds,while low concentration(<100 mg·L^(-1))of Cd stress did not significantly affect germination potential and germination rate,but resulted in a decrease in germination index and vigor index.2)Different concentrations of Cd stress inhibited shoot and root elongation,root vigor,and chlorophyll content accumulation in Brassica oleracea L.var.capitata L.seedlings,while biomass,soluble sugar,and soluble protein content showed inhibition only under Cd stress higher than 100 mg·L^(-1).In addition,Cd stress higher than 100 mg·L^(-1) also led to a significant increase in the relative conductivity of leaf blades of Brassica oleracea L.var.capitata L.seedlings.However,none of the different concentrations of Cd stress significantly affected malondialdehyde content and root-crown ratio.In summary,seed germination and seedling growth of Brassica oleracea L.var.capitata L.were tolerant to Cd stress below 100 mg·L^(-1),while Cd concentration higher than 100 mg·L^(-1) inhibited seed germination and seedling growth of Brassica oleracea L.var.capitata L.to a greater extent.
作者
裴云霞
倪天虹
张文君
张强
PEI Yunxia;NI Tianhong;ZHANG Wenjun;ZHANG Qiang(Eco-environmental Damage Judicial Expertise Center,Hubei Provincial Academy of Eco-environmental Sciences,Wuhan,Hubei 430072;Hubei Key Laboratory of Pollution Damage Assessment and Environmental Health Risk Prevention and Control,Wuhan,Hubei 430072;College of Horticulture,Xinyang Agriculture and Forestry University,Xinyang,Henan 464000)
出处
《北方园艺》
CAS
北大核心
2024年第19期9-16,共8页
Northern Horticulture
基金
湖北省生态环境科学研究院青年人才创新基金资助项目(2022-QNJJ-06)。
关键词
结球甘蓝
镉
种子萌发
幼苗生长
渗透调节物质
Brassica oleracea L.var.capitata L.
Cd
seed germination
seedling growth
osmotic adjustment substances
作者简介
第一作者:裴云霞(1993-),女,硕士,助理工程师,现主要从事生态学等研究工作。E-mail:674093700@qq.com;张强(1984-),男,博士,高级工程师,现主要从事环境生态学等研究工作。E-mail:d.ang@163.com。
