摘要
【目的】探究miR-141在动物机体糖脂代谢和骨代谢中的调控机制,为糖尿病合并骨质疏松症动物模型构建及相关基因表达调控机制研究提供依据。【方法】利用miRbase数据库获取不同物种miR-141成熟序列,通过BioEdit及WebLogo软件进行序列比对及保守性分析;利用PROMO及JASPAR在线软件对山羊miR-141启动子区转录因子结合位点进行预测;利用TargetScan和miRwalk数据平台对miR-141靶基因进行预测;利用DAVID和KOBAS在线软件对靶基因进行GO功能和KEGG通路富集分析;利用Networkanalyst数据库建立miRNA靶基因蛋白互作网络并绘制网络调控图;通过YM500V2在线数据库分析miR-141在山羊不同组织中的表达情况。【结果】miR-141成熟序列在不同物种间高度保守;山羊miR-141启动子区分布有转录因子C/EBPα、MyoD、YY1和Sp1等结合位点;选用不同数据库预测到Nfatc2、Egr2和Fasl等89个靶基因。GO功能富集分析发现,靶基因主要富集在RNA聚合酶Ⅱ启动子转录调控与转录DNA模板等生物过程、细胞核与细胞质等细胞组分、蛋白结合与DNA结合等分子功能。KEGG通路富集显示,靶基因主要富集在肌动蛋白细胞骨架的调节、细胞紧密连接、轴突引导、Hedgehog信号通路、cGMP-PKG信号通路与癌症中的microRNA等。miR-141靶基因蛋白互作分析显示,Nfatc2和Egr2基因由Fasl基因连接,构成与其他蛋白相对复杂的靶向关系,这些关键基因参与了与机体糖脂代谢及骨代谢相关的TGF-β、Hedgehog及Wnt/β-catenin等多条信号通路。miR-141在山羊多种组织中均有表达,其表达水平具有组织差异性。【结论】miR-141可通过靶向Nfatc2、Egr2和Fasl基因参与调节Hedgehog及Wnt/β-catenin等多条信号通路,并进一步调控山羊糖脂代谢、肌肉骨骼系统生理生化反应。
【Objective】This study was aimed to explore the regulatory mechanism of miR-141 in glucolipid metabolism and bone metabolism,and provide a basis for establishing animal model related to diabetes mellitus with osteoporosis and researching the regulation mechanism of miR-141 related gene expression.【Method】Mature miR-141 sequences of different species were obtained from miRbase database for sequence alignment and conservative analysis used BioEdit and WebLogo softwares.The PROMO and JASPAR online softwares were used to predict the transcription factor binding site of miR-141 in Capra hircus.The target genes of intersection were predicted by TargetScan and miRwalk online tools.GO function and KEGG pathway enrichment analysis of the predicted target genes were performed by DAVID and KOBAS online softwares.The functional protein interaction was analyzed with Networkanalyst database,meanwhile drawing the network regulation diagram.The YM500V2 online database was used to analyze the expression of miR-141 target genes in different tissues of Capra hircus.【Result】miR-141 mature sequences among different species were highly conservative.There were many transcription factor binding sites such as C/EBPα,MyoD,YY1 and Sp1 in the promoter region of miR-141 in Capra hircus.A total number of 89 target genes included Nfatc 2,Egr 2 and Fasl were predicted using different databases.GO function enrichment analysis results showed that the predicted target genes of miR-141 mainly enriched in the regulation of transcription from RNA polymeraseⅡpromoter and regulation of transcription-DNA-templated for biological process,nucleus and cytoplasm for cellular component,protein binding and DNA binding for molecular function.KEGG pathway enrichment analysis results showed that the target genes were significantly enriched in regulation of actin cytoskeleton,tight junction,axon guidance,Hedgehog signaling pathway,cGMP-PKG signaling pathway,microRNA in cancer and other signaling pathways.miR-141 target genes interaction analysis results revealed that Nfatc 2 and Egr 2 genes were connected by Fasl gene,which had more targeted relationships with other proteins,and involved in several signaling pathways,such as TGF-β,Hedgehog,Wnt/β-catenin and other pathways.miR-141 was expressed in different tissues of Capra hircus,and its expression exhibited tissue differences.【Conclusion】miR-141 participated in regulating some signaling pathways,represented by Hedgehog and Wnt/β-catenin signaling pathways with target genes Nfatc 2,Egr 2 and Fasl,and further regulated the glucolipid metabolism as well as physiological and biochemical reactions of musculoskeletal system in Capra hircus.
作者
马建青
宋鹏琰
杨清芳
孔建军
宋占锋
张亚丽
吴东蔚
徐增年
赵宁
周荣艳
吴占勇
MA Jianqing;SONG Pengyan;YANG Qingfang;KONG Jianjun;SONG Zhanfeng;ZHANG Yali;WU Dongwei;XU Zengnian;ZHAO Ning;ZHOU Rongyan;WU Zhanyong(Department of Orthopedic Laboratory,North China Medical&Health Group Xingtai General Hospital,Xingtai 054000,China;College of Animal Science and Technology,Tarim University,Alaer 843300,China;Xingtai Agriculture and Rural Affairs Bureau,Xingtai 054001,China;Third Hospital of Hebei Medical University,Shijiazhuang 050051,China;Deparment of Zoology,Hebei Medical University,Shijiazhuang 050017,China;College of Animal Science and Technology,Hebei Agricultural University,Baoding 071001,China)
出处
《中国畜牧兽医》
CAS
CSCD
北大核心
2024年第10期4211-4221,共11页
China Animal Husbandry & Veterinary Medicine
基金
邢台市重点研发计划项目(2023ZC156)
河北省自然科学基金(H2021108006)。
关键词
山羊
miR-141
靶基因
糖脂代谢
骨代谢
Capra hircus
miR-141
target gene
glucolipid metabolism
bone metabolism
作者简介
马建青,联系方式:E-mail:majianqing2006@126.com;通信作者:周荣艳,E-mail:rongyanzhou@126.com;通信作者:吴占勇,E-mail:xtsgkyy@126.com。
