摘要
BBM(BABY BOOM)转录因子在诱导植物体胚发生、促进细胞增殖与再生、提高遗传转化效率方面具有重要作用,常被应用于提高植物遗传转化效率。该研究利用生物信息学方法,系统鉴定了毛竹(Phyllostachys edulis)BBM家族成员,分析了系统进化关系、启动子调控元件、编码蛋白的理化性质、基因结构、蛋白质氨基酸保守序列,它们在染色体上的位置以及其蛋白二、三级结构;通过荧光定量PCR分析PeBBMs基因在不同组织器官中的表达模式。在毛竹中共鉴定出6个BBM家族基因,分布在6条不同染色体上。毛竹BBM蛋白编码486~699个氨基酸,等电点范围为6.31(PeBBM2)~8.92(PeBBM3)。毛竹BBM蛋白的二级结构预测表明:无规则卷曲和α-螺旋占比最高约80%。系统进化树表明,PeBBMs都聚类在AP2家族,亚细胞定位预测结果表明,毛竹BBM蛋白大部分位于细胞核,小部分位于细胞质。顺式作用元件分析表明,毛竹BBM的转录表达可能与非生物胁迫、光反应和转录调控相关。实时定量RT-PCR结果表明,PeBBM基因表达存在组织差异性,说明该基因家族不同成员在毛竹生长发育过程中发挥着不同的作用,其中PeBBMs在愈伤组织再生植株中相对表达量最高。推测PeBBMs可能参与毛竹愈伤组织再生过程的调控,该研究为深入研究毛竹BBM家族基因的功能奠定了基础。
BBM(BABY BOOM)transcription factors have important roles in inducing plant somatic embryogenesis,promoting cell proliferation and regeneration,and improving the efficiency of genetic transformation,and are often applied to improve the efficiency of plant genetic transformation.With the employment of the bioinformatics,this study identified the BBM family members of moso bamboo,and analyzed the phylogenetic relationships.The promoter regulatory elements,physicochemical properties of encoded proteins,gene structures,amino acid conserved sequences of proteins,positions in chromosomes,and their protein secondary and tertiary structures were analyzed;the expression pattern of moso bamboo BBM family members in different tissues and callus regeneration process was analyzed by real-time quantitative RT-PCR.A total of six BBM family genes were identified in Phyllostachys edulis,which were distributed on six different chromosomes.The BBM protein of moso bamboo encodes 486-699 amino acids,and its isoelectric point ranges from 6.31(PeBBM2)to 8.92(PeBBM3).Secondary structure prediction of BBM proteins showed that irregular coiled andα-helices accounted for up to about 80%.The phylogenetic tree showed that the PeBBMs were all clustered in the AP2 family,indicating that the BBMs of moso bamboo belonged to the AP2 family.The results of subcellular localization prediction showed that most of moso bamboo BBM proteins were located in the nucleus and the rest in the cytoplasm.Cis-acting element analysis indicated that the transcriptional expression of moso bamboo BBM might be associated with abiotic stress,light response and transcriptional regulation.Real-time quantitative RT-PCR results showed that there was tissue variability in the expression of the PeBBM gene,indicating that different members of the gene family played different roles in the growth and development of moso bamboo,with PeBBMs having the highest relative expression in callus regeneration process.It was hypothesized that PeBBMs might be involved in the process of moso banboo regeneration process.The results of this study laid a certain foundation for the in-depth study of the function of BBM family genes in moso bamboo.
作者
许若彤
周明兵
XU Ruotong;ZHOU Mingbing(Bamboo Industry Institute,Zhejiang A&F University,Hangzhou 311300,China)
出处
《中国细胞生物学学报》
CAS
CSCD
2024年第7期1377-1386,共10页
Chinese Journal of Cell Biology
基金
浙江自然科学基金(批准号:LZ24C160002)资助的课题。
关键词
BBM家族
表达分析
毛竹
再生
BBM family
expression pattern analysis
Phyllostachys edulis
regeneration
作者简介
通信作者:周明兵,Tel:13588152716,E-mail:zmbin@163.com。
