摘要
【目的】围绕玉米不同生育期根系土壤微生物的组成变化,探究鲜食玉米根系土壤微生物群落构成演化规律及其与环境因子的耦合关系。【方法】基于鲜食玉米大田试验,在鲜食玉米的播前期(PS)、拔节期(JS)、抽雄期(TS)和乳熟期(MS)进行根际土壤的采集及其理化性质的测定,并使用16S和ITS基因测序分析鲜食玉米不同生育期下根际土壤中微生物群落结构的特征。【结果】玉米不同生育期根系土壤细菌群落结构和多样性差异显著,而真菌群落组成在各生育期无显著变化。在拔节期,随土壤养分含量的显著降低,细菌变形菌门的相对丰度显著增加,而真菌子囊菌门的相对丰度则显著下降。在乳熟期,根系土壤细菌不同属间的正相关共生关系相较于其他生育期达到最高,而此时真菌不同属间的正相关共生关系则降至最低。结构方程模型分析结果显示,根际土壤细菌多样性与土壤pH、总磷和速效钾间存在正效应,而真菌多样性则与上述指标间存在负效应。【结论】土壤微生物群落结构在鲜食玉米生育期内发生显著改变,相比真菌,细菌更易受到外界环境的影响,微生物群落结构随外界环境的改变继而影响着鲜食玉米产量的构成。
[Objective]This experiment focused on the changes in the composition of soil microorganisms in the root system of maize at different growth stages,explored the evolution law of soil microbial community composition in maize roots and its coupling relationship with environmental factors.[Method]Based on the field experiment of fresh maize,the rhizosphere soil was collected and its physicochemical properties were determined at the pre-sowing stage(PS),jointing stage(JS),tasseling stage(TS),and milky stage(MS)of fresh maize.16S and ITS genesequencing wereusedtoanalyzethe characteristics ofmicrobial community structure intherhizosphere soiloffresh maize at diferent growth stages.[Result]There were significant differences in the structure and diversity of bacterial communities in the rhizosphere soil of maize at different growth stages,while the composition of fungal communities did not show significant changes at each growth stage.At the jointing stage,with a significant decrease in soil nutrient content,the relative abundance of Proteobacteria increased significantly,while the abundance of Ascomycota decreased significantly.During the milk ripening stage,the positive symbiotic relationship between diferent genera of bacteria in the rhizosphere soil reached its highest level compared to other growth stages,while the positive symbiotic relationship between different genera of fungi decreased to its lowest level.The structural equation modeling analysis results showed that there was a positive effect between rhizosphere soil bacterial diversity and soil pH,total phosphorus,and available potassium,while there was a negative effect between fungal diversity and the above indexes.[Conclusion]The structure of soil microbial community has significant changes during the growth period of fresh maize,bacteria are more susceptible to environmental influences compared with fungi,and the changes in microbial community structure with the environment also affect the formation of fresh maize yield.
作者
宗冬琳
周也富
胡小康
王弢
ZONG Dong-lin;ZHOU Ye-fu;HU Xiao-kang;WANG Tao(College of Agriculture and Biological Science,Dali University,Dali,Yunnan 671003,China;Co-Innovation Center for Cangshan Mountain and Erhai Lake Integrated Protection and Green Development of Yunnan Province,Dali University,Dali,Yunnan 671003,China)
出处
《西南农业学报》
CSCD
北大核心
2024年第8期1763-1770,共8页
Southwest China Journal of Agricultural Sciences
基金
国家自然科学基金项目(32201280)
云南省教育厅科学研究基金项目(2023Y1050)。
关键词
鲜食玉米
根际土壤微生物
生育期
土壤养分
高通量测序
Fresh maize
Rhizosphere soil microorganisms
Growing period
Soil nutrients
High throughput sequencing
作者简介
第一作者:宗冬琳(1997-),女,在读硕士,研究方向为农业生态学。E-mail:384646853@qq.com;通信作者:王弢(1987-),男,副教授,主要从事农业生态学研究。E-mail:wangtao12234@163.com。
