摘要
目的:研究滇鸡血藤红色素的最佳提取条件,考察其基本性质与环氧化酶-2(COX-2)抑制活性,为滇鸡血藤的临床用药及相关制剂开发提供参考。方法:以吸光度为指标,采用单因素试验和正交试验对滇鸡血藤红色素提取工艺中的液料比、浸提时间、乙醇体积分数进行优化,确定滇鸡血藤红色素的最佳提取工艺,对提取得到的红色素光、热、pH稳定性进行考察,进一步采用COX-2抑制剂筛选试剂盒考察滇鸡血藤红色素对COX-2的抑制作用。结果:在50%乙醇、液料比为110、浸提12 h的条件下提取的滇鸡血藤红色素吸光度最大;其在室温避光、pH 1~9条件下有较好的稳定性;滇鸡血藤红色素对COX-2有较好的抑制作用,半数抑制浓度(IC50)为2.04 ng·mL^(-1)(生药质量浓度)。结论:明确了滇鸡血藤红色素的最佳提取条件和稳定性条件,可为滇鸡血藤资源的综合开发利用与临床用药研究提供参考。
Objective:To optimize the extraction conditions of red pigment from Kadsurae Caulis,examine the basic properties and the inhibitory effect of the red pigment on cyclooxygenase-2(COX-2),and provide a reference for the clinical application of Kadsurae Caulis and the development of related preparations.Methods:With absorbance as the indicator,single factor and orthogonal experiments were carried out to optimize the liquid-to-solid ratio,extraction time,and ethanol concentration for the extraction of red pigment from Kadsurae Caulis.Furthermore,the light,thermal,and pH stability of the red pigment was examined,and the COX-2 Inhibitor Screening Kit was used to determine the inhibitory effect of the red pigment on COX-2.Results:The absorbance of the red pigment was the largest when the extraction was carried out with 50%ethanol and at the liquid-to-solid ratio of 110 for 12 h.The extracted pigment had good stability at room temperature and pH 1-9.Moreover,the extracted pigment inhibited the activity of COX-2,with the half maximal inhibitory concentration(IC50)of only 2.04 ng·mL^(-1)crude drug.Conclusion:This study optimized the extraction conditions and examined the stability of red pigment from Kadsurae Caulis.The extracted red pigment demonstrated strong inhibitory effect on COX-2.The findings lay a foundation for the comprehensive utilization and development of the resources and the clinical application of Kadsurae Caulis.
作者
乔子璇
姜丹
任广喜
吴菲
李萌
华国栋
刘春生
QIAO Zi-xuan;JIANG Dan;REN Guang-xi;WU Fei;LI Meng;HUA Guo-dong;LIU Chun-sheng(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China;Dongzhimen Hospital,Beijing University of Chinese Medicine,Beijing 100700,China)
出处
《中国现代中药》
CAS
2024年第9期1570-1577,共8页
Modern Chinese Medicine
基金
中央高校横向课题(2020110031001469)。
作者简介
通信作者:华国栋,主任药师,研究方向:临床中药和中药质量,Tel:010-84013381,E-mail:zhaojhuagd@126.com;通信作者:刘春生,教授,研究方向:药用植物与分子生药,Tel:010-53912100,E-mail:max_liucs@263.net。
