摘要
对来自广西伊岭岩风景区喀斯特溶洞真菌Metarhizium anisopliae NHC-M3-2的次级代谢产物进行提取研究。利用薄层色谱、高效液相色谱等对真菌次级代谢产物进行分离纯化,得到10个化合物,其中7-羟基-3-羟甲基-5,6-二甲基异铬-1-酮(1)与7-羟基-3,5,6-三甲基异铬-1-酮(2)为新结构的异香豆素类化合物,N-乙酰苯丙氨酸(3)、毛壳素J(4)、2-one-13-hydroxy-3,5,8,7(11)-eudesmatetraen-12,8-olide(5)、1H-吲哚-3-甲醛(6)、irpexolaceus B(7)irlactinL(8)、细胞松弛素K(9)、烟曲霉酸(10),共8个已知化合物。运用核磁共振谱、质谱等方法确定化合物结构,分别采用CCK-8法对化合物进行肿瘤细胞毒活性评价,结果显示,新化合物2对HepG2细胞的IC_(50)为29.83μmol·L^(-1),化合物9对HCT116细胞的IC_(50)为27.44μmol·L^(-1)。
Extracting extracts of secondary metabolites from the karst cave fungus Metarhizium anisopliae NHC-M3-2 from the Yilingyan Scenic Area in Guangxi.Ten compounds were isolated and purified from fungal secondary metabolites using thin-layer chromatography and high-performance liquid chromatography.7-Hydroxy-3-hydroxypropyl-5,6-dimethylisochrome-1-one(1)and 7-hydroxy-3,5,6-trimethyl-isochromen-1-one(2)were new isocoumarin compounds,N-acetyl phenylalanine(3),chaetosumin J(4),2-one-13-hydroxy-3,5,8,7(11)-eudesmatetraen-12,8-olide(5),1H-indole-3-carboxaldehyde(6),irpexolaceus B(7),irlactin L(8),cytochalasin K(9),and helvolic acid(10),a total of 8 known compounds.The structure of the compound was determined using methods such as NMR and mass spectrometry.The tumor cytotoxicity of the compound was evaluated using the CCK-8 method.The results showed that the IC_(50)of compound 2 on HepG2 cells was 29.83μmol·L^(-1),and compound 9 on HCT116 cells was 27.44μmol·L^(-1).
作者
伍荣香
郭杰兰
黄桦
廖婧婧
郝祎
孔凡栋
周丽曼
张朝军
WU Rong-xiang;GUO Jie-lan;HUANG Hua;LIAO Jing-jing;HAO Yi;KONG Fan-dong;ZHOU Li-man;ZHANG Chao-jun(Key Laboratory of Universities in Guangxi for Excavation and Development of Ancient Ethnomedicinal Recipes,Guangxi Collaborative Innovation Center for Chemistry and Engineering of Forest Products,Guangxi Key Laboratory of Chemistry and Engineering of Forest Products,Key Laboratory of Chemistry and Engineering of Forest Products,State Ethnic Affairs Commission,School of Chemistry and Chemical Engineering,Guangxi Minzu University,Nanning 530006,China;Zhejiang Kanglaite Pharmaceutical Limited Company,Hangzhou 310018,China)
出处
《药学学报》
CAS
CSCD
北大核心
2024年第9期2588-2593,共6页
Acta Pharmaceutica Sinica
基金
国家自然科学基金青年项目(82104034)
广西科技基地与人才专项(AD22035018)
广西自然科学基金项目(2021GXNSFBA220040)
广西民族大学校级引进人才科研启动项目(2020KJQD09,2021KJQD09)
广西民族大学自治区级大学生创新创业训练计划项目(S202210608141)
广西民族大学相思湖青年创新团队(2021RSCXSHQN01)
广西民族大学校级科研项目(2021MDKJ003).
作者简介
通讯作者:周丽曼,E-mail:zhouliman88@126.com;通讯作者:张朝军,E-mail:zhangchaojun@jemincare.com。
