摘要
目的探究水飞蓟素在创伤性颅脑损伤发生中的作用机制。方法选取24只SPF级昆明小鼠作为研究对象,采用随机数字表法分为假手术组(未建模)、水飞蓟素组(未建模给予水飞蓟素)、颅脑损伤组(建颅脑损伤模型,不干预)、颅脑损伤+水飞蓟素组(建颅脑损伤模型,给予水飞蓟素),每组6只。比较各组氧化应激反应、炎症反应指标、NLR家族Pyrin域蛋白3(NLRP3)炎症小体相关蛋白表达水平和神经功能。结果假手术组、水飞蓟素组超氧化物歧化酶(SOD)高于颅脑损伤+水飞蓟素组、颅脑损伤组,颅脑损伤+水飞蓟素组SOD高于颅脑损伤组,差异有统计学意义(P<0.05);假手术组、水飞蓟素组丙二醛(MDA)、肿瘤坏死因子-α(TNF-α)、白介素-1β(IL-1β)、白介素-6(IL-6)、NLRP3蛋白、Caspase-1-p20蛋白相对表达量低于颅脑损伤+水飞蓟素组、颅脑损伤组,颅脑损伤+水飞蓟素组各项指标低于颅脑损伤组,差异有统计学意义(P<0.05)。假手术组、水飞蓟素组小鼠颅脑创伤后3、7 d NSS评分低于颅脑损伤+水飞蓟素组、颅脑损伤组,颅脑损伤+水飞蓟素组低于颅脑损伤组,差异有统计学意义(P<0.05)。结论水飞蓟素在创伤性颅脑损伤治疗中可有效保护神经功能,可能与抑制氧化应激反应、炎症反应及NLRP3炎症小体活化有关。
Objective To explore the mechanism of silymarin in the occurrence of traumatic craniocerebral injury.Methods A total of 24 SPF Kunming mice were selected as the study object,and they were divided into sham operation group(not modeled),silymarin group(not modeled and given silymarin),craniocerebral injury group(craniocerebral injury model was established without intervention),craniocerebral injury+silymarin group(craniocerebral injury model was established and given silymarin)by random number table method,with 6 mice in each group.The oxidative stress response,inflammatory response index,expression level of NLR family pyrin domain protein 3(NLRP3)inflammasome related protein and neural function were compared among all groups.Results The superoxide dismutase(SOD)of sham operation group and silymarin group were higher than those of craniocerebral injury+silymarin group and craniocerebral injury group,and SOD of craniocerebral injury+silymarin group was higher than that of craniocerebral injury group,the differences were statistically significant(P<0.05).The relative expression levels of malonaldehyde(MDA),tumor necrosis factor-α(TNF-α),interleukin-1β(IL-1β),interleukin-6(IL-6),NLRP3 protein and Caspase-1-p20 protein in sham operation group and silymarin group were lower than those in craniocerebral injury+silymarin group and craniocerebral injury group,the indexes of craniocerebral injury+silymarin group were lower than those of craniocerebral injury group,and the differences were statistically significant(P<0.05).The NSS score of sham operation group and silymarin group at 3 and 7 days after craniocerebral trauma were lower than those of craniocerebral injury+silymarin group and craniocerebral injury group,and the NSS score of craniocerebral injury+silymarin group was lower than that of craniocerebral injury group,with statistical significances(P<0.05).Conclusion Silymarin can effectively protect nerve function in the treatment of traumatic craniocerebral injury,which may be related to inhibiting oxidative stress response,inflammatory response and activation of NLRP3 inflammasome.
作者
黄敏秋
方建喜
黄玲
何明球
袁饶饶
HUANG Minqiu;FANG Jianxi;HUANG Ling;HE Mingqiu;YUAN Raorao(Department of Neurosurgery,Guixi People's Hospital,Jiangxi Province,Yingtan 335400,China;Department of Internal Medicine,Guixi Center for Disease Control and Prevention,Jiangxi Province,Yingtan 335400,China;Department of Surgery,the Second Affiliated Hospital of Nanchang University,Jiangxi Province,Nanchang 330000,China)
出处
《中国当代医药》
CAS
2024年第24期9-13,共5页
China Modern Medicine
基金
江西省卫生健康委科技计划项目(SKJP220218103)。
关键词
创伤性颅脑损伤
小鼠
水飞蓟素
机制
Traumatic craniocerebral injury
Mice
Silymarin
Mechanism
作者简介
黄敏秋(1983-),男,江西崇仁人,副主任医师,研究方向:神经外科颅脑创伤患者预后影响因素。
