摘要
为提高石油污染土壤修复效果,该研究通过改进搅拌-交联方法制备陶粒-火山岩复合载体,同步进行微生物/漆酶的固定化,并结合SEM、BET、FTIR及土壤生物学特性等分析固定化载体性质及其在修复石油污染土壤中的差异。研究表明:陶粒-火山岩复合载体在石油烃吸附性能上较单一载体提升约50%,所制备的固定化载体比表面积提升3.1%~51.1%,孔容提升8.6%~50.9%;固定化微生物负载量达109cells/g,储存15 d活细胞比例最高达60.89%,固定化漆酶相对活性在循环利用5次后保持在19%以上,储存30 d为56%左右;土壤石油烃修复效果最高达58.98%(固定化菌-酶复合制剂),土壤脱氢酶、脲酶、过氧化氢酶及多酚氧化酶活性提升效果显著,且存在显著正相关关系;微生物结构分析表明添加固定化载体会使土壤微生物群落结构变化并出现优势物种,如铜绿假单胞菌属(Pseudomonas)、枝芽胞杆菌属(Virgibacillus)、大洋芽胞杆菌属(Oceanobacillus)。
In order to improve the remediation effect of oil contaminated soil,ceramic particle volcanic rock composite carrier was prepared by improving the stirring crosslinking method,and immobilization of microorganisms/laccase.Afterwards,SEM,BET,FTIR,and soil biological characteristics were combined to analyze the properties of immobilized carriers and their differences in the remediation of petroleum contaminated soil.One of the results shows that the composite carrier of ceramic particles and volcanic rocks has an improved petroleum hydrocarbon adsorption performance by about 50%compared to a single carrier.At the same time,the immobilized carrier prepared increased its specific surface area by 3.1%~51.1%and pore volume by 8.6%~50.9%.The immobilized microbial load reaches the order of 109 cells/g.The proportion of living cells stored for 15 days reached 60.89%.The relative activity of immobilized laccase remains above 19%after 5 cycles of recycling,and about 56%after 30 days of storage.The highest remediation effect of soil petroleum hydrocarbons is 58.98%(immobilized bacteria enzyme complex preparation).Soil dehydrogenase,urease,catalase and polyphenol oxidase were significantly improved.And there is a significant positive correlation with petroleum hydrocarbons.The microbial structure analysis showed that adding immobilized carrier would change the soil microbial community structure and lead to the emergence of dominant species,including Pseudomonas,Virgibacillus and Oceanobacillus.
作者
刘佳璇
李法云
李晓桐
周纯亮
LIU Jiaxuan;LI Fayun;LI Xiaotong;ZHOU Chunliang(School of Ecological Technology and Engineering,Shanghai Institute of Technology,Shanghai 201418,China;Institute of Beautiful China and Ecological Civilization,University Think Tank of Shanghai Municipality,Shanghai 201418,China;Shanghai Engineering Research Center of Urban Ecological Technology,Shanghai 201418,China)
出处
《环境科学与技术》
CAS
CSCD
北大核心
2024年第7期112-123,共12页
Environmental Science & Technology
基金
国家重点研发计划项目课题:长效广谱生物修复载体与固定化菌剂制备技术(2020YFC1808802)。
关键词
无机载体
微生物
漆酶
固定化
石油污染土壤
inorganic carrier
microorganisms
laccase
immobilization
oil contaminated soil
作者简介
刘佳璇(1999-),男,硕士研究生,主要研究方向为有机污染土壤修复技术,(电子信箱)2997477656@qq.com;通讯作者:李法云,男,教授,(电子信箱)lnecology@163.com。
