摘要
炔雌醇(17α-乙炔基雌二醇,EE2)是常用的雌激素药物与养殖业激素添加剂,可导致严重的环境雌激素污染。制备和应用高效稳定的固定化菌剂,有助于实现环境炔雌醇污染的有效去除。本研究利用海藻酸钙法对前期分离的雌激素降解香茅醇假单胞菌(Pseudomonas citronellolis)SJTE-3进行固定,制备降解性能更稳定的固定化菌剂。正交试验确定了菌剂的最佳固定化条件是3%海藻酸钠溶液、4%CaCl2溶液和2 mL的OD600为10.0的菌液量。优化的固定化菌剂对Tris-HCl液体培养基中10μg·mL-1的炔雌醇的去除率可达78.8%,较游离态菌剂的去除率提高了23.0%。同时,该固定化菌剂对模拟污水中不同浓度炔雌醇(5~50μg·mL-1)的去除率均可达到78%,显著高于游离态菌剂。此外,此固定化菌剂可多次重复使用,稳定降解时间长,重复使用10次后,其炔雌醇去除率仍可达53%。固定化香茅醇假单胞菌SJTE-3菌剂降解效能稳定高效,环境适应性高,具有实际修复应用的潜能。
Ethinylestradiol(17α-ethynylestradiol, EE2)is an estrogenic drug and hormone additive that is commonly used in aquaculture and causes serious environmental estrogen pollution. The preparation and application of stable immobilized bacterial agents can achieve the effective removal of environmental EE2 pollution. In this study, the calcium alginate method was used to fix estrogen-degrading Pseudomonas citronellolis SJTE-3 in order to create immobilized bacterial agents with more stable degradation performance. The results of the orthogonal tests demonstrated that the best immobilization conditions were 3% sodium alginate solution, 4% CaCl2 solution, and 2 mL of bacterial solution with an OD600 of 10.0. The degradation efficiency of the optimized immobilized bacterial agent for an EE2 concentration of10 μg·mL-1 in the Tris-HCl medium reached 78.8%, which was approximately 23.0% higher than that of the free bacterial agent.Meanwhile, the removal rate of EE2(5~50 μg·mL-1)in the simulated sewage by the immobilized bacterial agent was approximately 78%,which was significantly higher than that of the free bacterial agent. In addition, the immobilized bacterial agent could be used repeatedly with a long and stable degradation time. After the 10th use, the EE2 removal rate was still 53%. The immobilized Pseudomonas citronellolis SJTE-3 bacterial agent has a stable and efficient degradation capability, high environmental adaptability, and application potential for actual remediation.
作者
王雁秋
彭万里
梁如冰
WANG Yan-qiu;PENG Wan-li;LIANG Ru-bing(School of Life Sciences and Biotechnology,State Key Laboratory of Microbial Metabolism,Shanghai Jiao Tong University,Shanghai 200240,China)
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2020年第8期1803-1810,共8页
Journal of Agro-Environment Science
基金
上海市自然科学基金项目(19ZR1475500)。
关键词
香茅醇假单胞菌SJTE-3
炔雌醇
固定化菌剂
海藻酸钙
微生物降解
Pseudomonas citronellolisSJTE-3
17α-ethinylestradiol
immobilized bacterial agent
calcium alginate
microbial degradation
作者简介
王雁秋(1995—),女,浙江江山人,硕士研究生,从事环境微生物降解研究。E-mail:wyq501194864@163.com;通信作者:梁如冰,E-mail:icelike@sjtu.edu.cn。
