摘要
试验旨在对高产表面活性素菌株枯草芽孢杆菌BYA-KC-4的发酵条件进行优化,筛选出最佳的生产表面活性素的条件。试验通过单因素试验确定影响产表面活性素的因素水平。采用Box-Behnken中心组合设计,进一步优化了发酵条件参数。结果表明,在最佳发酵条件下,即33℃、初始pH值6.8、接种量4.8%、转速201 r/min,表面活性素的产量达到了560.25 mg/L,相比优化前的425.36 mg/L,提高了1.32倍。研究表明,试验可为枯草芽孢杆菌BYA-KC-4产表面活性素工业化生产提供参考。
The experiment aimed to optimize the fermentation conditions for the high-yielding surfactin-producing strain BacillussubtilisBYA-KC-4 and to screen for the best conditions for surfactin production.The experiment determined the levels of factors affecting surfactin production through single-factor tests.The Box-Behnken central composite design was used to further optimize the fermentation condition parameters.The results showed that under the optimal fermentation conditions,namely a temperature of 33℃,an initial pH value of 6.8,an inoculum volume of 4.8%,and a rotation speed of 201 r/min,the yield of surfactin reached 560.25 mg/L.Compared with the 425.36 mg/L before optimization,it increased by 1.32 times.The study indicates that the experiment can provide a reference for surfactin production by BacillussubtilisBYA-KC-4 and for industrial production.
作者
李高强
张旭斌
牛俊轲
刘永立
张锋
高原源
李梦洁
贵文娟
LI Gao-qiang;ZHANG Xu-bin;NIU Jun-ke;LIU Yong-li;ZHANG Feng;GAO Yuan-yuan;LI Meng-jie;GUI Wen-juan
出处
《饲料研究》
CAS
北大核心
2024年第14期107-112,共6页
Feed Research
关键词
表面活性素
枯草芽孢杆菌
响应面优化
发酵条件
surfactin
Bacillussubtilis
response surface optimization
fermentation conditions
作者简介
第一作者:李高强,硕士,研究方向为益生菌发酵及应用。
