摘要
以“四季”青绿苔草的胚性愈伤组织为试验材料,选用秋水仙素为诱导剂,采用秋水仙素浸泡处理法进行诱变处理,研究了不同的秋水仙素浓度和不同的处理时间下诱导“四季”青绿苔草多倍体的最佳条件。通过流式细胞术、根尖染色体计数的方法对加倍植株进行倍性鉴定,并进行叶片气孔特征和植株表型特征分析,以期为“四季”青绿苔草的多倍体诱导及苔草新品种培育提供参考依据。结果表明:在秋水仙素浓度为0.10%,处理时间为48 h时诱导率最高,该条件下的诱导率可达65%。通过植株表型与气孔特征比较,加倍后的植株与二倍体植株有明显的差异,表现为叶型宽阔、株高降低、气孔变大等特点。
Taking the embryogenic callus of Carer breviculmis‘Siji'as the test material,selecting colchicine as the inducer,and using the colchicine soaking method for mutagenesis treatment.The optimal conditions for inducing polyploidy of Carer breviculmis‘Siji'were under different colchicine concentrations and different treatments times.The ploidy of doubled plants was identified by flow cytometry and root tip chromosome counting,and leaf stomatal characteristics and plant phenotypic characteristics were analyzed,in order to provide reference for the polyploid induction of Carea breviculmis‘Siji'and the cultivation of new Carer varieties.The results showed that the induction rate was the highest when the colchicine concentration was 0.1o%and the treatment time was 48 hours,and the induction rate under these conditions could reach 65%.Comparing the plant phenotype and stomatal characteristics,it was found that the doubled plants were significantly different from the diploid plants,which were characterized by broad leaf shape,reduced plant height,and enlarged stomatal pores.
作者
贾明
董双慧
潮洛濛
范希峰
武菊英
岳跃森
JIA Ming;DONG Shuanghui;CHAO Luomeng;FAN Xifeng;WU Juying;YUE Yuesen(Institute of Grassland,Flowers and Landscape Ecology,Beijing Academy of Agriculture and Forestry Sciences/North China Key Laboratory of Urban Agriculture,Ministry of Agriculture and Rural Affairs,Beijing 100097;School of Ecology and Environment,Inner Mongolia University,Hohhot,Inner Mongolia 01021)
出处
《北方园艺》
CAS
北大核心
2024年第15期52-59,共8页
Northern Horticulture
基金
北京市农林科学院科技创新专项资助项目(KJCX20230811,KJCX20230119)
现代农业产业技术体系北京市创新团队资助项目(BAIC09-2023)。
作者简介
第一作者:贾明(1998-),女,硕士研究生,研究方向为苔草多倍体育种。E-mail:jiaming.00@foxmail.com;岳跃森(1981-),男,博士,副研究员,现主要从事草资源与育种等研究工作。E-mail:yysen2008@sina.com。
