摘要
在组织培养条件下,以'开远'滇杨幼嫩茎段为实验材料,用秋水仙素作为诱导剂,对'开远'滇杨进行多倍体诱导,比较不同预培养时间、 不同处理浓度以及不同处理时间下秋水仙素的诱导效果.结果表明:'开远'滇杨幼嫩茎段在分化培养基上预培养3d后,再在添加有40mg/L秋水仙素的分化培养基中处理3d,其诱导效果最佳,变异率可达50.79%.'开远'滇杨多倍性材料'同质化'处理的最佳次数为6次;对所得的多倍体材料进行流式细胞术检测,其倍性为四倍.
The colchicine was used to induce female diploid Populus yunnanensis‘Kaiyuan’based on the tissue culture,polyploid induction was carried out on the cultivar,and the induction effects of colchicine were compared under different pre-culture time,different treatment concentrations and different treatment time.The results showed that optimal induction effect would be obtained when the young stem of P.yunnanensis‘Kaiyuan’were pre-cultured on differentiation medium for 3 days,then treated with the differentiation medium supplemented with 40mg/L colchicine for 3 days.The variation rate would be 50.79%.The number of optimal homogenization of the polyploidy materials was six times.Based on the analysis of flow cytometry,the ploid of polyploidy materials was tetraploid.
作者
叶鹏
李显煌
辛亚龙
瞿绍宏
董章宏
徐剑
李贤忠
辛培尧
YE Peng;LI Xian-huang;XIN Ya-long;QU Shao-hong;DONG Zhang-hong;XU Jian;LI Xian-zhong;XIN Pei-yao(Key Laboratory for Forest Resources Conservation and Use in the Southwest Mountains of China,Ministry of Education,Kunming Yunnan 650224,P.R.China;Forestry Bureau of Jiangchuan District,Yuxi Yunnan 652600,P.R.China)
出处
《西部林业科学》
CAS
北大核心
2019年第4期114-118,共5页
Journal of West China Forestry Science
基金
云南省教育厅科学研究基金项目(2017YJS087)
云南省教育厅科学研究基金重大专项项目(ZD2015014)
西南林业大学科研基金项目“不同基因型滇杨多倍体新种质的选育”
关键词
'开远'滇杨
雌株
多倍体种质
诱导
Populus yunnanensis‘Kaiyuan’Dode
female plant
polyploid
作者简介
第一作者简介:叶鹏(1995-),男,硕士研究生,主要从事林木遗传育种方面的研究。E-mail:1484438673@qq.com;通讯作者:辛培尧(1975-),男,教授,主要从事林木遗传育种方面的研究。E-mail:xpytgyx@163.com
