摘要
采用乳化溶剂挥发法制备纳米粒,选取PLGA为载体材料,PVA溶液为水相,黄芩苷为主药,以包封率为评价指标,进行单因素考察和响应面优化,筛选出黄芩苷PLGA纳米粒的最佳制备工艺,并考察其体外释放度行为。最后,通过开展细胞试验,对黄芩苷PLGA纳米粒进行体外药效学评价。结果表明,最优工艺及处方制备出的黄芩苷PLGA纳米粒,体外释放具有缓释性,对肝癌细胞具有显著抑制作用。
Nanoparticles were prepared using emulsion-solvent evaporation method,with PLGA as the carrier material,PVA solution as the aqueous phase,and baicalin as the main drug.The encapsulation efficiency was used as the evaluation indicator,and a single-factor investigation and response surface optimization were conducted to screen the optimal preparation process for baicalin-loaded PLGA nanoparticles and examine their in vitro release behavior.Finally,cell experiments were conducted to evaluate the in vitro pharmacodynamics of baicalin-loaded PLGA nanoparticles.The results showed that the baicalin-loaded PLGA nanoparticles prepared using the optimal process and formula exhibited sustained release in vitro and demonstrated significant inhibitory effects on liver cancer cells.
作者
汪洁
顾雪梅
范丹丹
王丽媛
吕志阳
陈璟
WANG Jie;GU Xue-mei;FAN Dan-dan;WANG Li-yuan;LV Zhi-yang;CHEN Jing(Hanlin College of Nanjing University of Chinese Medicine,Jiangsu Taizhou 225300,China)
出处
《广州化工》
CAS
2024年第13期21-24,共4页
GuangZhou Chemical Industry
基金
2024年江苏高校青蓝工程优秀教学团队资助项目
2023年江苏省大学生创新创业项目(202313981004Y、202313981011Y)
泰州市科技支撑项目(TS202232、TS202325)。
关键词
黄芩苷
PLGA
纳米粒
体外释放度
肝癌
baicalin
PLGA
nanoparticles
in vitro release behavior
liver cancer
作者简介
第一作者:汪洁,女,硕士研究生,讲师,主要研究方向为药物新剂型与新技术;通讯作者:顾雪梅,女,硕士研究生,讲师,主要研究方向为药物新剂型与新技术。
