摘要
目的建立同时测定车前草药材中12种元素的电感耦合等离子体质谱(ICP-MS)法,并进行化学计量学分析。方法射频功率为1800 W,等离子体气流量为15 L/min,载气流量为1 L/min,采样深度为12 mm,辅助器流量为1 L/min,雾化室温度为2.0℃,测量模式为动能歧视(KED)模式,碰撞气体积流量为6.0 mL/min,进样冲洗时间为35 s,蠕动泵泵速为50 r/min,扫描3次。根据3个产地15批车前草药材样品中12种元素的含量测定结果,建立特征图谱,并进行主成分分析。结果镉(Cd)、铅(Pb)、铜(Cu)、钠(Na)、锌(Zn)、钾(K)、铝(Al)、汞(Hg)、钙(Ca)、镁(Mg)、砷(As)、铁(Fe)质量浓度分别在0~100 ng/mL、0~100 ng/mL、0~500 ng/mL、0~500 ng/mL、0~1000 ng/mL、0~500 ng/mL、0~500 ng/mL、0~20 ng/mL、0~500 ng/mL、0~1000 ng/mL、0~100 ng/mL、0~1000 ng/mL范围内与仪器响应值线性关系良好(r≥0.9992,n=6);精密度、重复性、稳定性试验结果的RSD均小于5%;平均加样回收率为82.72%~91.10%,RSD为1.55%~4.69%(n=6)。15批车前草12种无机元素的平均含量分别为0.36,1.58,9.16,10.51,54.71,9.35,34.86,0.16,16.81,112.64,1.08,100.04 mg/kg。3个产地车前草药材中12种元素特征图谱的峰形基本相似。Mg、Fe、Zn、Al是车前草药材的特征元素;15批车前草药材样品的质量存在一定差异,其中贵州毕节所产的质量较好。结论建立的ICP-MS法操作简单、结果准确,可用于车前草药材中12种元素的含量测定;特征图谱及主成分分析可为车前草药材的开发利用和质量控制提供参考。
Objective To establish an inductively coupled plasma-mass spectrometry(ICP-MS)method for simultaneous determination of 12 elements in Plantaginis Herba,and to perform chemometric analysis.Methods The radio frequency power was 1800 W,the plasma gas flow rate was 15 L/min,the carrier gas flow rate was 1 L/min,the sampling depth was 12 mm,the auxiliary flow rate was 1 L/min,the atomization chamber temperature was 2.0℃,the measurement mode was kinetic energy discrimination(KED)mode,the collision gas flow rate was 6.0 mL/min,the injection flushing time was 35 s,the peristaltic pump speed was 50 r/min,and the scanning frequency was three times.Based on the contents of 12 elements in 15 batches of Plantaginis Herba from three producing areas,the characteristic spectra were established,and the principal component analysis was performed.Results The linear ranges of cadmium(Cd),lead(Pb),copper(Cu),sodium(Na),zinc(Zn),potassium(K),aluminum(Al),mercury(Hg),calcium(Ca),magnesium(Mg),arsenic(As)and iron(Fe)were in the range of 0-100 ng/mL,0-100 ng/mL,0-500 ng/mL,0-500 ng/mL,0-1000 ng/mL,0-500 ng/mL,0-500 ng/mL,0-20 ng/mL,0-500 ng/mL,0-1000 ng/mL,0-100 ng/mL,0-1000 ng/mL(r≥0.9992,n=6)respectively.The RSDs of precision,repeatability and stability tests were all lower than 5%.The average recovery rates of the above 12 elements were in the range of 82.72%-91.10%,with RSDs of 1.55%-4.69%(n=6).The average contents of 12 inorganic elements in 15 batches of Plantaginis Herba were 0.36,1.58,9.16,10.51,54.71,9.35,34.86,0.16,16.81,112.64,1.08,100.04 mg/kg,respectively.The peak shapes of characteristic spectra of 12 elements in Plantaginis Herba from three different producing areas were similar.Mg,Fe,Zn and Al were the characteristic elements of Plantaginis Herba.The quality of 15 batches of Plantaginis Herba was different,and that in Bijie,Guizhou was the best.Conclusion The established ICP-MS method is simple and accurate,which can be used for the content determination of 12 elements in Plantaginis Herba.The characteristic spectra and principal component analysis can provide a reference for the development,utilization and quality control of Plantaginis Herba.
作者
卫蓓蓓
陈维中
邬倩
WEI Beibei;CHEN Weizhong;WU Qian(The 900th Hospital of the Joint Logistics Support Force of PLA,Fuzhou,Fujian,China 350000)
出处
《中国药业》
CAS
2024年第16期78-81,共4页
China Pharmaceuticals
关键词
车前草
电感耦合等离子体质谱法
无机元素
特征图谱
主成分分析
含量测定
Plantaginis Herba
inductively coupled plasma-mass spectrometry
inorganic element
characteristic spectra
principal component analysis
content determination
作者简介
第一作者:卫蓓蓓,女,主管药师,研究方向为药物质量研究与分析,(电子信箱)1054761986@qq.com;通信作者:陈维中,男,大学本科,主任药师,研究方向为药物质量分析及中药分析,(电子信箱)1145750405@qq.com。
