摘要
目的对临床医学检验过程中的血液细胞检验结果展开调查,以探讨保障检验质量的方法。方法60例行血液细胞检验患者为研究对象,回顾分析不同存放时间、抗凝剂比例的检验结果,并统计不同存放时间的溶血事件发生情况。结果1∶10000抗凝剂比例的血红蛋白、血小板计数、白细胞计数、红细胞计数分别为(144.31±10.69)g/L、(184.49±24.94)×10^(9)/L、(10.33±1.89)×10^(9)/L、(5.41±0.91)×10^(12)/L,比1∶5000抗凝剂比例的(106.22±7.09)g/L、(134.01±20.69)×10^(9)/L、(6.31±1.01)×10^(9)/L、(3.92±0.61)×10^(12)/L更高(P<0.05)。存放2、4 h的血红蛋白、血小板计数、白细胞计数、红细胞计数比存放0.5 h更高(P<0.05);存放4 h的血红蛋白、血小板计数、白细胞计数比存放2 h更高(P<0.05)。存放0.5、2、4 h均出现溶血事件,分别为1、3、7例,占比分别为1.67%、5.00%、11.67%。存放4 h的溶血事件发生率高于存放0.5 h(χ^(2)=4.821,P=0.028<0.05)。结论抗凝剂比例、血液标本存放时间均会给血液细胞检验结果带来影响,而且标本长时间存放还会引发溶血事件。血液细胞检验过程中,需严格控制抗凝剂比例、存放时间,加强对每个环节的控制,以提高检验结果的准确度。
Objective To investigate the results of blood cell testing during clinical medical testing and explore methods to ensure the quality of testing.Methods 60 patients who underwent blood cell tests were selected as the survey subjects.The test results under different storage time and anticoagulant ratios were retrospectively analyzed,and the occurrence of hemolysis events under different storage time were statistically analyzed.Results Hemoglobin,platelet count,white blood cell count and red blood cell count at an anticoagulant ratio of 1∶1000 were(144.31±10.69)g/L,(184.49±24.94)×10^(9)/L,(10.33±1.89)×10^(9)/L,and(5.41±0.91)×10^(12)/L,which were higher than(106.22±7.09)g/L,(134.01±20.69)×10^(9)/L,(6.31±1.01)×10^(9)/L,and(3.92±0.61)×10^(12)/L at an anticoagulant ratio of 1∶5000(P<0.05).The hemoglobin,platelet count,white blood cell count and red blood cell count with a storage time of 2 and 4 h were higher than those with a storage time of 0.5 h(P<0.05).The hemoglobin,platelet count,and white blood cell count with a storage time of 4 h were higher than those with a storage time of 2 h(P<0.05).Hemolysis events occurred after storage for 0.5,2 and 4 h,with 1,3 and 7 cases,accounting for 1.67%,5.00%and 11.67%,respectively.The incidence of hemolysis events after storage for 4 h was higher than that after storage for 0.5 h(χ^(2)=4.821,P=0.028<0.05).Conclusion Anticoagulant ratio and the storage time of blood specimens can both affect the results of blood cell tests,and long-term storage of specimens can also lead to hemolysis events.During the process of blood cell testing,it is necessary to strictly control the proportion and storage time of anticoagulants,strengthen the control of each step,and improve the accuracy of the test results.
作者
俞淼
王祎
YU Miao;WANG Yi(Department of Clinical Laboratory,Beijing First Hospital of Integrated Traditional Chinese and Western Medicine,Beijing 100026,China)
出处
《中国实用医药》
2024年第14期98-100,共3页
China Practical Medicine
关键词
临床医学检验
血液细胞检验
存放时间
检验质量
Clinical medical testing
Blood cell testing
Storage time
Inspection quality
作者简介
通讯作者:王祎。
