摘要
为探明常用生物农药与具有生防潜力的昆虫病原真菌莱氏绿僵菌Nr5772的相容性,从而为两者联合使用防治农业害虫提供依据,采用室内菌丝生长速率法测定了常用生物源杀虫剂多杀菌素、藜芦碱、苦参碱、除虫菊素、鱼藤酮、印楝素对莱氏绿僵菌的影响。结果表明,苦参碱与莱氏绿僵菌的相容性最好,前者对后者的抑制作用较弱,抑制中浓度EC_(50)为324.12mg/L;多杀菌素对莱氏绿僵菌的EC_(50)为175.83mg/L,两者相容性也较好;除虫菊素、印楝素对莱氏绿僵菌的菌丝生长具有一定抑制作用,对应的EC_(50)分别为48.57、8.99 mg/L;鱼藤酮和藜芦碱对莱氏绿僵菌的菌丝生长表现出很强的抑制作用,EC_(50)值分别为3.28 mg/L和1.68 mg/L,远低于这两种农药的田间常用浓度40 mg/L和12.5 mg/L,与莱氏绿僵菌的相容性差。由此判断,莱氏绿僵菌可与苦参碱或多杀菌素混用以提升对害虫的防效,但不适合与鱼藤酮或藜芦碱混用。
The compatibility of Metarhizium rileyi Nr5772 with commonly used biopesticides was explored so as to provide a basis for the suitable mixing of the two for the control of agricultural pests.The effects of biopesticides spinosad,veratrine,matrine,pyrethrin,rotenone,and azadirachtin on the growth of M.rileyi were determined by measuring the mycelium growth rate indoor.The results showed that matrine exhibited the most favorable compatibility with M.rileyi,with an EC_(50)value of324.12 mg/L.Spinosad also had good compatibility,with an EC_(50)value of 175.83 mg/L.In contrast,rotenone and veratrine exhibited poor compatibility with M.rileyi,exerting a strong inhibitory effect on the mycelium growth of M.rileyi,with EC_(50)values of 3.28 mg/L and 1.68 mg/L,respectively.The results showed that M.rileyi can be mixed with matrine and spinosad to enhance the efficacy.However,it was not compatible with the addition of rotenone and veratrine.
作者
张希鹏
武广安
王煜坤
刘守柱
Zhang Xipeng;Wu Guang'an;Wang Yukun;Liu Shouzhu(Agricultural Science and Engineering School,Liaocheng University,Liaocheng Shandong 252000,China)
出处
《中国植保导刊》
北大核心
2024年第6期14-17,39,共5页
China Plant Protection
基金
山东省自然科学基金项目(ZR2020MC127)
聊城大学大学生创新创业训练计划项目(CXCY2022219)。
关键词
莱氏绿僵菌
生物农药
相容性
室内毒力
Metarhizium rileyi
biopesticides
compatibility
indoor toxicity
作者简介
张希鹏,硕士研究生,研究方向为园林植物害虫生物防治。E-mail:m17852266701@163.com;通讯作者:刘守柱,博士,副教授,主要从事昆虫病理及害虫生物防治研究。E-mail:liushouzhu@lcu.edu.cn。