摘要
目的探讨脑胶质瘤患者组织中环状RNA circSMAD2(hsa_circ_0047603和hsa_circ_0108525)的水平及临床意义。方法收集本院手术切除并经病理证实的脑胶质瘤组织85例,以32例正常脑组织为参照采用实时定量PCR检测脑胶质瘤组织中hsa_circ_0047603和hsa_circ_0108525水平,分层分析hsa_circ_0047603和hsa_circ_0108525水平与脑胶质瘤临床病理特征的关系,结合随访数据和Cox比例风险回归模型评估hsa_circ_0047603和hsa_circ_0108525水平在脑胶质瘤预后中的价值。结果脑胶质瘤组织的hsa_circ_0047603和hsa_circ_0108525水平均高于正常组织(P<0.001)。hsa_circ_0047603和hsa_circ_0108525水平与病理类型、WHO分级、瘤周水肿和浸润深度均有关(P<0.05),而与性别和年龄无关(P>0.05)。单因素分析显示组织hsa_circ_0047603和hsa_circ_0108525水平与脑胶质瘤的总生存期(OS)有关(P<0.05),其中hsa_circ_0047603、hsa_circ_0108525低水平者的中位OS均为47.0个月,优于高水平者的25.5和35.0个月;多因素分析显示hsa_circ_0047603高表达(HR=2.149,95%CI:1.283~3.598,P=0.006)和hsa_circ_0108525高表达(HR=1.844,95%CI:1.022~3.327,P=0.028)均是脑胶质瘤预后不良的独立因素。结论circSMAD2的上调可能对预测脑胶质瘤患者的临床结果有价值,尤其是对那些病理分级高的患者,有作为该肿瘤生物标志物的潜能。
Objective To investigate the the level and clinical significance of circular RNA circSMAD2(hsa_circ_0047603 and hsa_circ_0108525)in the tissues of glioma patients.Methods Eighty-five cases of glioma tissues surgically removed and pathologically confirmed in our hospital were collected.Real-time quantitative PCR was used to detect the levels of hsa_circ_0047603 and hsa_circ_0108525 in the glioma tissues using 32 normal brain tissues as a reference.Stratified analysis was conducted to investigate the relationship between levels of hsa_circ_0047603 and hsa_circ_0108525 and the clinical pathological characteristics of glioma.The value of hsa_circ_0047603 and hsa_circ_0108525 levels in the prognosis of glioma was evaluated by combining follow-up data and Cox proportional hazards regression model.Results Levels of hsa_circ_0047603 and hsa_circ_0108525 in glioma tissues were higher than those in normal tissue(P<0.001).Levels of hsa_circ_0047603 and hsa_circ_0108525 were correlated with pathological types,WHO grading,peritumoral edema and depth of infiltration(P<0.05),but not with gender and age(P>0.05).Univariate analysis showed that the levels of hsa_circ_0047603 and hsa_circ_0108525 were associated with overall survival(OS)of gliomas(P<0.05).The median OS of low level individuals was 47.0 months for hsa_circ_0047603 and hsa_circ_0108525,better than that of high level individuals at 25.5 and 35.0 months.Multivariate analysis showed that up-regulated levels of hsa_circ_0047603(HR=2.149,95%CI:1.283-3.598,P=0.006)and hsa_circ_108525(HR=1.844,95%CI:1.022-3.327,P=0.028)were independent factors for poor prognosis in glioma.Conclusion Upregulation of circSMAD2 may have value in predicting clinical outcomes in patients with glioma,especially for those with high pathological grading,and has the potential to serve as a biomarker for this tumor.
作者
丁玉召
符静波
郑喜胜
李长力
张元峰
DING Yuzhao;FU Jingbo;ZHENG Xisheng;LI Changli;ZHANG Yuanfeng(Department of Neurosurgery,Nanyang Second People's Hospital,Nanyang 473000,China)
出处
《临床肿瘤学杂志》
CAS
2024年第4期267-271,共5页
Chinese Clinical Oncology
作者简介
通讯作者:郑喜胜,E-mail:1402089205@qq.com。
