摘要
目的 分离并鉴定鸽源产酸克雷伯菌,探究其生物学特性及致病性。方法 剖检甘肃某信鸽棚发病鸽,观察并采集病变组织,通过观察病菌形态、生化试验、16S rRNA PCR鉴定和序列分析实现对致病菌的分离鉴定,结合组织病理学观察、药敏试验和致病性试验对分离菌株进行耐药性和致病性研究。结果 病鸽肝、肺、心等多器官出血,肝脏发黄且质地脆,肺脏发生化脓;从病鸽中成功分离到一株革兰氏阴性、短杆状菌株,麦康凯琼脂培养基上为粉红色光滑、湿润、圆形菌落,靛基质试验阳性;16S rRNA扩增序列与MN330093.1同源性达100.00%,表明病鸽感染产酸克雷伯菌,并命名为GS-BY-GG;产酸克雷伯菌GS-BY-GG对青霉素、氨苄西林和呋喃唑酮等10种药物耐药,对氟苯尼考、美罗培南和庆大霉素等5种药物敏感;组织病理学观察可见病鸽多器官出血,肝脏细胞排列不规则、多处可见棕黄色色素沉积,气管黏膜上皮广泛细胞坏死和脱落、黏膜层可见大量炎性细胞浸润;接种SPF雏鸡显示分离菌具有很强的致病性。结论 本研究成功分离了鸽源产酸克雷伯菌GS-BY-GG,研究结果为产酸克雷伯的临床诊断和防治提供数据支撑。
The biological characteristics and pathogenicity of Klebsiella oxytoca isolated from sick carrier pigeons in Gansu province were explored by morphological observations,biochemical testing,16S rRNA PCR analysis,and RNA sequencing.The drug resistance and pathogenicity of the isolated strains were studied by histopathological observation,drug susceptibility testing,and pathogenicity analysis.The livers,lungs,hearts,and other organs of the sick pigeons were bleeding.In addition,the livers were yellow and brittle,and the lungs were purulent.A Gram-negative,short,rod-shaped bacterium was successfully isolated from the sick pigeon.Pink,smooth,moist,and round colonies grew on MacConkey’s agar.The result of the indigo matrix test was positive.The homology between the amplified 16S rRNA sequence and MN330093.1 was 100.00%,indicating that the sick pigeon was infected with K.oxytoca.The strain was named GS-BY-GG.K.Oxytoca GS-BY-GG was resistant to 10 drugs,including penicillin,ampicillin,and fura zolidone,and sensitive to 5 others,which included florfenicol,meropenem,and gentamicin.Histopathological observation showed bleeding in multiple organs.The liver cells were irregularly arranged with brown-yellow pigmentation.Extensive cell necrosis and exfoliation were observed in the trachea and mucosal epithelium,with inflammatory cell infiltration in the mucosal layer.The isolates were highly pathogenic in specific pathogen-free chickens.These findings provide support for the clinical diagnosis and control of K.oxytoca GS-BY-GG.
作者
李韦
李赟辉
金有顺
巴旭丽
张淮瑜
韩涛
李兆才
周继章
LI Wei;LI Yun-hui;JIN You-shun;BA Xu-li;ZHANG Huai-yu;HAN Tao;LI Zhao-cai;ZHOU Ji-zhang(State Key Laboratory for Animal Disease Control and Prevention,College of Veterinary Medicine,Lanzhou University,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou 730000,China;Corllege of Veterinary Medicine Northwest A&F University,Yangling 712100,China;Xian Xiangxiaren Co.,Ltd,Lantian 710500,China;Gansu Province Research Center for Basic Disciplines of Pathogen Biology,Lanzhou 730046,China)
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2024年第6期512-519,共8页
Chinese Journal of Zoonoses
基金
国家重点研发计划专项(No.2022YFC2304001)。
关键词
产酸克雷伯菌
信鸽
分离鉴定
耐药情况
组织病理学
Klebsiella oxytoca
racing pigeon
isolation and identification
drug resistance
histopathology
作者简介
通讯作者:周继章,Email:zhoujz@lzu.edu.cn,ORCID:0000-0003-4764-9328。
