摘要
目的探讨当归多糖对低氧环境中骨髓间充质干细胞(BMSC)成骨分化潜能的影响。方法将BMSC随机分为空白组、低氧组、当归多糖组。使用OriCellTM成人BMSC成骨诱导分化完全培养基对3组细胞进行培养,其中当归多糖组的培养基含有50μg/mL的当归多糖。对空白组BMSC进行常规培养(21%氧浓度),将低氧组与当归多糖组BMSC置于6%氧浓度环境中进行培养。培养14 d后,观察3组的BMSC形态学变化和钙盐沉积情况,检测3组BMSC的成骨分化相关蛋白(骨桥蛋白和骨钙素)表达水平,以及成骨分化关键转录因子RUNT相关转录因子2(RUNX2)表达情况。结果与空白组相比,低氧组BMSC的骨钙素、骨桥蛋白、RUNX2的表达水平均降低(P<0.05),高密度钙结节及钙盐沉积减少。与低氧组相比,当归多糖组BMSC骨钙素、骨桥蛋白、RUNX2的表达升高(P<0.05),高密度钙结节及钙盐沉积增多。结论低氧环境可以抑制BMSC的成骨分化潜能,而当归多糖能够有效维持低氧环境中BMSC成骨分化潜能的稳定。
Objective To explore the effect of Angelica sinensis polysaccharide on osteogenic differentiation potential of bone marrow stem cell(BMSC)in hypoxia.Methods BMSC was randomly divided into blank group,hypoxia group,or Angelica sinensis polysaccharide group.OriCellTM complete medium of adult BMSC osteogenesis⁃induced differentiation was used to perform culture in cells of the three groups,therein medium of the Angelica sinensis polysaccharide group contained 50μg/mL Angelica sinensis polysaccharide.BMSC routine culture was given to the blank group(21%oxygen concentration),and BMSC of the hypoxia group and the Angelica sinensis polysaccharide group was placed in an environment with 6%oxygen concentration to perform culture.After 14 days of culture,BMSC morphological change and calcium salt deposition condition were observed in the three groups.BMSC osteogenic differentiation⁃associated proteins(osteopontin and osteocalcin)expressions,and BMSC osteogenic differentiation key transcription factor Runt⁃related transcription factor 2(RUNX2)expression were detected in the three groups.Results Compared with the blank group,the hypoxia group exhibited decreased expressions of osteocalcin,osteopontin,and RUNX2(P<0.05),and presented as reduced high⁃density calcium nodules and calcium salt deposition.Compared with the hypoxia group,the Angelica sinensis polysaccharide group yielded elevated BMSC osteocalcin,osteopontin,and RUNX2 expressions(P<0.05),and presented as increased high⁃density calcium nodules and calcium salt deposition.Conclusion Hypoxia can inhibit BMSC osteogenic differentiation potential,and Angelica sinensis polysaccharide can effectively maintain stability of BMSC osteogenic differentiation potential in hypoxia.
作者
蔡林利
朱霞
陈雁
CAI Linli;ZHU Xia;CHEN Yan(Department of Integrated Traditional Chinese and Western Medicine,West China Hospital,Sichuan University,Chengdu 610041,Sichuan,China;Infectious Disease Center,West China Hospital,Sichuan University,Chengdu 610041,Sichuan,China)
出处
《广西医学》
CAS
2024年第5期727-730,共4页
Guangxi Medical Journal
基金
四川省科技计划项目(2019YFS0231)。
关键词
骨髓间充质干细胞
当归多糖
低氧环境
成骨分化
Bone marrow stem cell
Angelica sinensis polysaccharide
Hypoxia
Osteogenic differentiation
作者简介
第一作者:蔡林利,硕士,主治医师,研究方向为中西医结合治疗呼吸系统疾病;通信作者:朱霞,硕士,副主任医师,研究方向为病毒性肝炎的基础与临床研究。
