摘要
为探索杜仲叶多糖的超声辅助水提工艺及其对自由基的体外抗氧化性能,文章在单因素试验基础上,利用响应面BBD优化超声辅助水提杜仲叶多糖,且采用透析膜对多糖提取物进行透析纯化,最后研究了多糖提取物对羟基和DPPH自由基的清除能力。结果表明:该超声辅助水提工艺在优化后的条件下,即液料比20 mL/g、夹层温度62℃、超声功率500 W、超声时间65 min时,测得的多糖提取率为2.66%,多糖提取物中多糖含量为23.21%,经过透析纯化后其多糖含量为58.75%;杜仲叶多糖对羟基和DPPH自由基都有较好的清除作用,但更有利于羟基自由基的清除;透析纯化处理不仅能改善多糖纯度,还可以提高多糖对羟基和DPPH自由基的清除能力,尤其在清除DPPH自由基时表现明显。该分离纯化工艺高效绿色,可直接用于杜仲叶多糖的提取,促进杜仲多糖在饲料、食品、医药等领域的开发应用。
To explore the ultrasound-assisted water extraction of polysaccharides from Eucommia ulmoi-des leaves and corresponding antioxidant activity against free radicals in vitro,the response surface meth-odology BBD was used to optimize the extraction on the basis of single factor tests,and the polysaccha-ride extract was purified by dialysis,followed by the determination of hydroxyl and DPPH radicals-scavenging by polysaccharide extracts in vitro.The results showed that the extraction yield of polysaccha-ride is 2.66%,under the revised optimal conditions,i.e.,liquid-solid ratio of 20 mL/g,interlayer tem-perature of 62℃,ultrasonic power of 500 W,ul-trasonic time of 65 min.The polysaccharide con-tent in the polysaccharide extract is 23.21%and 58.75%before and after dialysis purification,re-spectively.The polysaccharide extract showed a better scavenging effect on both hydroxyl and DPPH radicals,but it was more favorable to scav-enge hydroxyl radicals.Dialysis purification can not only improve purity of polysaccharides,but also increase the corresponding scavenging ability on hydroxyl and DPPH radicals,especially in the scavenging of DPPH radicals.The separation and puri-fication processes are efficient and green,and can be directly used for the extraction of polysaccharides from Eucommia ulmoides leaf,promoting the development and application of Eucommia ulmoides polysac-charide in feed,food,medicine and other fields.
作者
王润平
李云萍
路小彬
贺银菊
张宏福
WANG Runping;LI Yunping;LU Xiaobin;HE Yinju;ZHANG Hongfu(School of Chemistry and Chemical Engineering,Qiannan Normal University for Nationalities,Ethnic Medicinal Plant Resources Development Engineering Research Center of Colleges and Universities in Guizhou Province,Guizhou Qiannan 558000,China;State Key Laboratory of Animal Nutrition,Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing 100193,China)
出处
《饲料工业》
CAS
北大核心
2024年第14期87-95,共9页
Feed Industry
基金
国家自然科学基金[21868029]
贵州省科技厅学术新苗项目[黔科合平台人才2020QNSYXM05]
贵州省教育厅平台滚动支持项目[黔财教202178]
贵州省科技厅自然科学基金[黔科合基础20201Y047]。
关键词
杜仲叶
多糖
超声辅助
BBD
透析
自由基
Eucommia ulmoides leaves
polysaccharides
ultrasound-assisted
BBD
dialysis
free radical
作者简介
王润平,博士,副教授,研究方向为天然产物的提取分离;通讯作者:贺银菊,副教授。
