摘要
以湖北百合为试材,采用RT-PCR方法克隆MYB基因,利用DNAMAN 8.0软件、ExPaSy-ProParam软件、NetPhos 3.1 Server等工具,研究了MYB基因所编码蛋白的结构以及功能,以期为开展湖北百合相关MYB转录因子的研究提供参考依据。结果表明:LhrMYB1、LhrMYB15蛋白为稳定蛋白、碱性蛋白、亲水性蛋白,LhrMYB12蛋白为稳定蛋白、酸性蛋白、亲水性蛋白;LhrMYB16蛋白为不稳定蛋白、碱性蛋白、亲水性蛋白。LhrMYB1与芒果中MYB3-like亲缘关系最近(XM 044620835),LhrMYB12与百合杂交品种亲缘关系最近(MK182389),LhrMYB15与百合杂交品种division I中MYB15like亲缘关系最近(LC218141),LhrMYB16与百合杂交品种division I中MYB16-1亲缘关系最近(LC218139)。
Taking Lilium henryi Baker as the test material,the MYB gene was cloned using RT-PCR method.The structure and function of the protein encoded by the MYB gene were studied using tools such as DNAMAN 8.0,ExPaSy-ProParam,and NetPhos 3.1 Server,in order to provide reference for the study of MYB transcription factors relused to Lilium henryi Baker.The results showed that LhrMYB1 and LhrMYB15 proteins were stable proteins,alkaline proteins,and hydrophilic proteins,while LhrMYB12 protein was stable protein,acidic protein,and hydrophilic protein;LhrMYB16 protein was an unstable protein,alkaline protein,and hydrophilic protein.LhrMYB1 had the closest relationship with MYB3-like in mango(XM 044620835),LhrMYB12 had the closest relationship with lily hybrid variety(MK182389),LhrMYB15 had the closest relationship with lily hybrid variety division I MYB15like(LC218141),and LhrMYB16 had the closest relationship with lily hybrid variety division I MYB16-1(LC218139).
作者
董然
金鸽
林强
韩东洋
钟慧烨
张秀海
DONG Ran;JIN Ge;LIN Qiang;HAN Dongyang;ZHONG Huiye;ZHANG Xiuhai(Liupao Tea Modern Industry College,Wuzhou University,Wuzhou,Guangxi 543002;College of Forestry and Grassland Science,Jilin Agricultural University,Changchun,Jilin 130118;Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097;College of Resources and Environment,Xizang Agriculture and Animal Husbandry College,Nyingchi,Xizang 860000)
出处
《北方园艺》
CAS
北大核心
2024年第12期42-51,共10页
Northern Horticulture
基金
北京市农林科学院创新能力专项资助项目(KJCX20230106)
北京市农林科学院科研创新平台建设资助项目(PT2023-2)
现代农业产业技术体系北京市创新团队资助项目(BAIC09-2023)。
作者简介
第一作者:董然(1966-),女,博士,教授,现主要从事经济植物资源与利用等研究工作。E-mail:1836630983@qq.com;张秀海(1972-),男,博士,研究员,现主要从事百合分子育种等研究工作。E-mail:zhangxiuhai@baafs.net.cn.
