摘要
目的研究2型糖尿病大鼠中亲环蛋白A(cyclophilin A,CyPA)和X盒结合蛋白1(X-box binding protein 1,XBP1)的表达情况。方法建立1型糖尿病和2型糖尿病SD大鼠模型,同期饲养普通饮食和高脂饮食大鼠,酶联免疫吸附试验(enzyme-linked immunosorbent assay,ELISA)检测4组大鼠CyPA和XBP1在外周血的表达情况,苏木精-伊红染色(hematoxylin-eosin staining,HE染色)观察4组大鼠冠状动脉组织形态学改变,免疫组化法检测4组大鼠下肢股动脉组织CyPA和XBP1的表达情况。结果2型糖尿病大鼠血清CyPA表达水平较普食组、高脂喂养组和1型糖尿病组升高,差异有统计学意义(t分别为-7.626、3.677、5.040,P<0.01)。2型糖尿病组大鼠血清XBP1表达水平较普食组、高脂喂养组降低,差异有统计学意义(t分别为4.208、2.997,P分别为0.001、0.013),较1型糖尿病组降低,差异无统计学意义(t=1.843,P=0.084)。HE染色未见4组大鼠冠状动脉发生明显脂肪化改变。免疫组化法检测结果显示,CyPA在2型糖尿病组大鼠动脉组织中表达水平最高;XBP1在高脂喂养组大鼠动脉组织中表达水平最高,在2型糖尿病组大鼠动脉组织中表达水平最低。结论高糖、高脂环境与大鼠外周血和动脉组织CyPA和XBP1表达水平具有相关性,其中高糖、高脂联合作用的影响相较单纯高糖或单纯高脂环境强。
Objective To investigate the expression of cyclophilin A(CyPA)and X-box binding protein 1(XBP1)of type 2 diabetes mellitus in rats.Methods SD rat models of type 1 diabetes mellitus and type 2 diabetes mellitus were established,and rats with normal diet and high fat diet were fed at the same time.The expression of CyPA and XBP1 in peripheral blood of rats in the four groups was detected by enzyme-linked immunosorbent assay(ELISA),the morphological changes of coronary artery in the four groups were observed by hematoxylin-eosin staining(HE staining),and the expressions of CyPA and XBP1 in femoral artery of lower limbs of rats in the four groups were detected by immunohistochemistry.Results The expression level of CyPA in peripheral blood of rats with type 2 diabetes mellitus was higher than that in normal diet group,high fat feeding group and type 1 diabetes mellitus group,and the difference was statistically significant(t were-7.626,3.677,5.040,P<0.01).The expression level of XBP1 in peripheral blood of rats in type 2 diabetes mellitus group was lower than that in normal diet group and high fat feeding group,and the difference was statistically significant(t were 4.208,2.997;P were 0.001,0.013).The expression level of XBP1 in peripheral blood of rats in type 2 diabetes mellitus group was lower than that in type 1 diabetes mellitus group,and the difference was not statistically significant(t=1.843,P=0.084).HE staining did not show significant fatty changes in the coronary arteries of the four groups.Immunohistochemistry showed that the expression level of CyPA was the highest in the arterial tissues of rats with type 2 diabetes mellitus group,while the expression level of XBP1 was the highest in the arterial tissues of rats with high fat feeding group and the lowest in the arterial tissues of rats with type 2 diabetes mellitus group.Conclusion High glucose and high fat was correlated with the expression levels of CyPA and XBP1 in in peripheral blood and arterial tissue of rats,and the combined effect of high glucose and high fat was stronger than that of high glucose or high fat alone.
作者
杨雯雯
王宁
YANG Wenwen;WANG Ning(The First Affiliated Hospital of Xinjiang Medical University,Xinjiang 830000,China)
出处
《医学研究杂志》
2024年第6期131-135,共5页
Journal of Medical Research
基金
省部共建中亚高发病成因与防治国家重点实验室开放课题(SKL-HIDCA-2020-19)。
作者简介
通信作者:王宁,电子信箱:13999994126@163.com。
