摘要
为研究壮药蛇尾草高效液相(HPLC)指纹图谱及抗氧化活性间的谱效关系,建立了蛇尾草药材高效液相指纹图谱,采用1,1-二苯基-2-三硝基苯肼法(DPPH)和铁离子还原能力法(FRAP)对蛇尾草抗氧化活性进行评价,结合聚类分析、主成分分析和偏最小二乘分析对蛇尾草抗氧化活性进行谱效分析。结果表明,以3号峰(毛蕊花糖苷)为参照峰,确定了11个共有峰,大部分批次蛇尾草的相似度>0.900;通过聚类分析和主成分分析,可将22批样品分为两类;不同产地间蛇尾草毛蕊花糖苷含量在0.38%~4.82%;22批蛇尾草抗氧化活性良好,共有峰1、2、3(毛蕊花糖苷)、6、8、10、11与DPPH自由基清除率呈正相关,1、2、3(毛蕊花糖苷)、6、10号峰与铁离子还原能力呈正相关。建立的蛇尾草HPLC指纹图谱可靠、准确,可为蛇尾草抗氧化活性物质的筛选及质量控制提供参考。
Aiming to study the relationship between high performance liquid ehromatography(HPLC)fingerprint and antioxidant ac-tivity,the HPLC fingerprint of Pogonostemon auricularius(L.)Hassk.was established.Combing with Cluster Analysis,Principal Component Analysis,and Partial Least Squares Regression Analysis,the antioxidant activity of Pogonostemon auricularius(L.)Has-sk.was evaluated by 1,1-diphenyl-2-trinitrophenylhydrazine(DPPH)and ferric reducing ability of plasma(FRAP)methods.The re-sults showed that eleven common peaks were identified with peak No.3(verbascoside)as the reference peak,and the similarity of most batches of Pogonostemon auricularius(L.)Hassk.was greater 0.900.Through cluster analysis and principal component analysis,twenty-two batches of samples could be divided into two categories.The content of verbascoside in Pogonostemon auricularius(L.)Hassk.from different producing areas ranged from 0.38%to 4.82%.The antioxidant activity of twenty-two lots of Pogonostemon auricu-larius(L.)Hassk.was good.Peaks 1,2,3(verbascoside),6,8,10,and 11 were positively correlated with DPPH free radical scav-enging rate,peaks 1,2,3(verbascoside),6,and 10 were positively correlated with iron ion reducing ability.The established HPLC fingerprint was reliable and accurate,which could provide reference for the screening and quality control of antioxidant active sub-stances of Pogonostemon auricularius(L.)Hassk.
作者
马雯芳
吴剑丽
张秀丽
蓝昌斌
甘洁雪
周华锋
王剑
MA Wen-fang;WU Jian-i;ZHANG Xiu-i;LAN Chang-bin;GAN Jie-xue;ZHOU Hua-feng;WANG Jian(Faculty of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530001,China)
出处
《湖北农业科学》
2024年第5期84-90,共7页
Hubei Agricultural Sciences
基金
壮瑶药协同创新中心项目(桂教科研[2013]20号)
广西壮瑶药重点实验室项目(桂科基字[2014]32号)
广西重点学科壮药学项目(桂教科研[2013]16号)
广西协同创新中心壮瑶药协同创新中心科研项目(ZYXT[2015]-13)。
关键词
蛇尾草
指纹图谱
聚类分析
主成分分析
抗氧化活性
谱效关系
偏最小二乘回归分析
Pogonostemon auricularius(L.)Hassk.
fingerprint
cluster analysis
principal component analysis
antioxidant activi-ty
spectrum effect relationship
Partial Least Squares Regression Analysis
作者简介
马雯芳(1983-),女,广西南宁人,教授,博士,主要从事中药品种鉴定与品质评价研究工作,(电话)0771-4953513(电子信箱)109348793@qq.com;通信作者:王剑(1981-),讲师,硕士,主要从事中医药数据挖掘的研究工作,(电话)0771-4953513(电子信箱)43582538@qq.com。
