摘要
采用多种分离纯化方法,包括硅胶、凝胶、薄层色谱以及半制备高效液相等;并结合瘤背石磺生化特性、NMR数据分析及文献比较,对化合物进行结构鉴定。从瘤背石磺中分离鉴定出12个化合物,经鉴定结构为Cyclo-(L-Pro-L-Val)(1)、Cyclo-(Pro-Ile)(2)、Cyclo-(L-Pro-D-Leu)(3)、Tyramine(4)、Cyclo-(L-ProL-Phe)(5)、2-哌啶酮(6)、Cyclo-(L-Ala-L-Pro)(7)、尿嘧啶核糖核苷(8)、尿嘧啶(9)、胸腺嘧啶(10)、2'-甲氧基尿嘧啶核苷(11)、胸苷(12)。化合物(11~6,11)均为首次从瘤背石磺中分离得到。
Onchidium verruculatum,a characteristic resource in the South China Sea,was extracted and isolated by using a variety of separation and purification methods,including silica gel,Sephadex LH-20,thin layer chromatography and semi-prepared HPLC.The structures of the compounds were identified by combining their biochemical characteristics,NMR data analysis and reference comparison.Twelve compounds were isolated from Onchidium verruculatum,and their structures were identified as Cyclo-(L-Pro-L-Val)(1),Cyclo-(Pro-Ile)(2),Cyclo-(L-Pro-D-Leu)(3),Tyramine(4),Cyclo-(L-Pro-L-Phe)(5),2-Piperidone(6),Cyclo-(L-Ala-L-Pro)(7),uracil ribonucleoside(8),uracil(9),thymine(10),2'-methoxyuracil nucleoside(11),thymidine(12).Compounds(1-6,11)were isolated for the first time from Onchidium verruculatum.
作者
邓雨婷
黄兹宝
刘雪菲丹
曹明玉
周媛媛
董琳
张小坡
DENG Yuting;HUANG Zibao;LIU Xuefeidan;CAO Mingyu;ZHOU Yuanyuan;DONG Lin;ZHANG Xiaopo(Pharmacy College,Hainan Medical University,Haikou,Hainan 571199,China)
出处
《热带生物学报》
2024年第3期368-372,共5页
Journal of Tropical Biology
基金
海南省重点研究发展计划(ZDYF2022SHFZ037)
国家自然科学基金项目(82060778)。
作者简介
第一作者:邓雨婷(1999-),女,海南医学院2021级硕士研究生。E-mail:2634452320@qq.com;通信作者:张小坡(1982-),男,教授,博士,硕士生导师。研究方向:南药黎药研究。E-mail:z_xp1412@163.com。
