摘要
目的探讨桂药生精胶囊对少精子症(肾精亏虚型)患者的精子浓度(SC)、前向运动精子比例(PR)、PR+非前向运动精子比例(NP)、精子总数、精子脂肪酸合成酶(Fas)信使RNA(mRNA)、脂肪酸合成酶配体(FasL)mRNA表达水平及精浆Fas蛋白、FasL蛋白表达水平的影响。方法选取2019年10月至2020年3月就诊于广西中医药大学附属瑞康医院男性科门诊的少精子症(肾精亏虚型)患者60例,随机分为对照组和治疗组,每组30例。对照组采用左卡尼汀治疗,治疗组在对照组的基础上联合桂药生精胶囊治疗,12周为1个疗程。观察治疗前后两组患者的SC、PR、PR+NP、精子总数、精子Fas mRNA、FasL mRNA和精浆Fas蛋白、FasL蛋白表达水平。结果治疗组总有效率比对照组高(P<0.05)。治疗前两组患者SC、PR、PR+NP和精子总数比较,差异均无统计学意义(P>0.05)。治疗后两组患者精子SC、PR、PR+NP和精子总数均比治疗前高(P<0.05),且治疗组患者精子SC、PR和精子总数高于对照组(P<0.05)。治疗前两组患者精浆Fas和FasL蛋白表达水平比较,差异均无统计学意义(P>0.05)。治疗后两组精浆Fas蛋白表达水平低于治疗前(P<0.05),且治疗组Fas蛋白表达水平低于对照组(P<0.05)。治疗前两组患者精子Fas mRNA和FasL mRNA表达水平比较,差异均无统计学意义(P>0.05)。治疗后两组患者精子Fas mRNA和FasL mRNA表达水平低于治疗前(P<0.05),且治疗组Fas mRNA表达水平低于对照组(P<0.05)。结论桂药生精胶囊能明显提高少精子症(肾精亏虚型)患者的SC、PR、精子总数,降低精子Fas mRNA及精浆Fas蛋白的表达水平。
Objective To investigate the effects of Guiyao Shengjing capsule on sperm concentration(SC),the proportion of progressively motile sperm(PR),PR+non-progressively motile sperm(NP),total sperm count and sperm fatty acid synthase(Fas)messenger RNA(mRNA),fatty acid synthase ligand(FasL)mRNA expression levels and in seminal plasma Fas protein and FasL protein expression levels in the patients with oligospermia(kidney essence deficiency).Methods A total of 60 patients with oligozoospermia(kidney essence deficiency)in the Department of Andrology,Ruikang Hospital Affiliated to Guangxi University of Traditional Chinese Medicine from October 2019 to March 2020 were randomly divided into control group and treatment group,with 30 cases in each group.The control group was treated with L-carnitine,and the treatment group was treated with Guiyao Shengjing capsule on the basis of the control group,12 weeks as a course of treatment.The SC,PR,PR+NP,total sperm count and the expression levels of sperm Fas mRNA,FasL mRNA,Fas protein,FasL protein in seminal plasma were observed before and after treatment.Results The total effective rate of the treatment group was higher than that of the control group(P<0.05).Before treatment,there were no significant differences in SC,PR,PR+NP and total sperm count between the two groups(P>0.05).After treatment,the sperm SC,PR,PR+NP and total sperm count in the two groups were higher than those before treatment(P<0.05),and the sperm SC,PR and total sperm count in the treatment group were higher than those in the control group(P<0.05).There was no significant difference in the expression levels of Fas protein and FasL protein in seminal plasma between the two groups before treatment(P>0.05).After treatment,the expression level of Fas protein in the seminal plasma of the two groups was lower than that before treatment(P<0.05),and the expression level of Fas protein in the treatment group was lower than that in the control group(P<0.05).There was no significant difference in the expression of sperm Fas mRNA and FasL mRNA between the two groups before treatment(P>0.05).After treatment,the expression levels of sperm Fas mRNA and FasL mRNA in the two groups were lower than those before treatment(P<0.05),and the expression level of sperm Fas mRNA in the treatment group was lower than that in the control group(P<0.05).Conclusion Guiyao Shengjing Capsule can significantly improve the SC,PR and total sperm count and reduce the expression levels of Fas mRNA and Fas protein in the seminal plasma of oligozoospermia patients.
作者
戴芳
唐亚平
买鹏宇
张泽朝
朱闽
DAI Fang;TANG Yaping;MAI Pengyu;ZHANG Zezhao;ZHU Min(Department of Clinical Laboratory,Ruikang Hospital Affiliated to Guangxi University of Traditional Chinese Medicine,Nanning,Guangxi 530011,China;College of Basic Medical Sciences,Guangxi University of Traditional Chinese Medicine,Nanning,Guangxi 530200,China;Department of Science and Technology,Ruikang Hospital Affiliated to Guangxi University of Traditional Chinese Medicine,Nanning,Guangxi 530011,China;Department of Andrology,Ruikang Hospital Affiliated to Guangxi University of Traditional Chinese Medicine,Nanning,Guangxi 530011,China)
出处
《检验医学与临床》
CAS
2024年第11期1543-1547,共5页
Laboratory Medicine and Clinic
基金
广西壮族自治区中医药管理局科研课题(GZZC2019112)。
关键词
桂药生精胶囊
少精子症
肾精亏虚型
脂肪酸合成酶
脂肪酸合成酶配体
Guiyao Shengjing capsule
oligospermia
kidney essence deficiency type
fatty acid synthase
fatty acid synthase ligand
作者简介
戴芳,女,主任技师,主要从事临床检验方向的研究;通信作者:朱闽,E-mail:hnf22@126.com。
