摘要
对栀子黄色素的纯化工艺条件进行优化。以闽东福鼎的栀子为原料,通过大孔树脂的筛选,研究洗脱剂浓度和流速对吸附过程的影响,确定大孔树脂纯化栀子黄色素的新工艺。结果表明,HPD-400型大孔树脂对栀子黄色素的吸附量大,解析率高,纯化效果较好;最佳柱层析条件为洗脱剂质量分数80%,洗脱流速1.0 mL/min。将洗脱液浓缩,真空干燥即得高纯度栀子黄色素,所得黄色素OD_(238 nm/440 nm)比值由1.86降到0.27,色价达到510。为纯化栀子黄色素提供了一种好方法。
To optimize the purification process of gardenia yellow pigment.The gardenia of Fuding in eastern Fujian was used as raw material,and a new method for purification of gardenia yellow pigment with macroporous resin was determined.The effect of concentration and flow rate of eluent on the adsorption process was studied through resin screening.The results showed that the macroporous resin HPD-400 had large adsorption capacity,high resolution and good purification effect for gardenia yellow pigment.The optimum column chromatographic conditions were 80%concentration of eluent and 1.0 mL/min elution flow rate.The high purity gardenia yellow pigment was obtained by concentrating the eluent and vacuum drying.The ratio of OD_(238 nm/440 nm)of the yellow pigment was reduced from 1.86 to 0.27,and the color value was 510.This paper developed a good method for purification of gardenia yellow pigment.
作者
罗义发
潘斌
周三女
刘丽清
田妍基
施满容
LUO Yifa;PAN Bin;ZHOU Sannv;LIU Liqing;TIAN Yanji;SHI Manrong(Ningde Vocational and Technical College,Fu'an,Fujian 355000,China)
出处
《农产品加工》
2024年第7期36-38,43,共4页
Farm Products Processing
基金
福建省中青年教师教育科研项目立项项目(科技类)(JAT171135)
宁德市科技计划项目(20170072)
宁德职业技术学院育苗基金项目(YM2016ZR03)。
关键词
栀子黄色素
大孔树脂
纯化
gardenia yellow pigment
macroporous resin
purification
作者简介
罗义发(1977-),男,硕士,讲师,工程师,研究方向为微生物转化、植物中有效成分的提取分离、微生物发酵;通讯作者:施满容(1965-),女,本科,教授,研究方向为植物植生与生物技术专业教学。
