摘要
该实验借助AB-8树脂联用C18分离纯化牡丹籽粕中新橙皮苷,并进行生物活性探究,为开发相关产品提供依据。借助吸附动力学模型筛选了6种大孔树脂(AB-8、X-5、DM301、CAD-40、D101和NKA),AB-8树脂的吸附/解析能力优于其他5种;优化AB-8的分离条件为上样速度8 BV/h、上样量为60 mL、洗脱剂为60%(体积分数)乙醇溶液、洗脱流速为5 mL/min;抗氧化活性测试表明1 mg/mL新橙皮苷对于DPPH自由基、超氧阴离子自由基和ABTS阳离子自由基的清除能力分别为83.23%、81.05%和72.03%;250 mg/mL新橙皮苷可以通过提高细胞内抗氧化活性来减少细胞内活性氧含量;实验表明,2000 mg/mL新橙皮苷对于K562的抑制率为92.49%,500 mg/mL新橙皮苷可以有效抑制K562分裂的G1期;分子动力学模拟结果表明新橙皮苷可以通过结合B淋巴细胞瘤-2基因、细胞分裂蛋白激酶2生成氢键作用来抑制K562增殖。该实验从牡丹籽粕中分离出了活性物质新橙皮苷,为科学认识牡丹籽粕废弃物提供理论基础。
In this study,neohesperidin in peony seed meal was isolated and purified by AB-8 resin combined with C18,and its biological activity was explored,which provided a basis for the development of related products.Six macroporous resins(AB-8,X-5,DM301,CAD-40,D101,and NKA)were screened by the adsorption kinetics model,and the adsorption/analytical capacity of AB-8 resin was superior to the other five.The optimal separation conditions for AB-8 were that the loading rate was 8 BV/h,the loading volume was 60 mL,the eluent was 60%(volume fraction)ethanol-aqueous solution,and the elution flow rate was 5 mL/min.The antioxidant activity of 1 mg/mL neohesperidin was 83.23%,81.05%,and 72.03%for DPPH free radicals,·O 2-,and ABTScationic radicals,respectively.250 mg/mL of new hesperidin can improve the antioxidant activity in the cell activity to reduce intracellular reactive oxygen species content.Experiments showed that the inhibition rate of 2000 mg/mL of new hesperidin on K562 was 92.49%,and 500 mg/mL of new hesperidin could effectively inhibit the K562 G1 phase of division.Molecular dynamics simulation results showed that neohesperidin inhibited the proliferation of K562 through hydrogen bonding with B-cell lymphoma-2 and cyclin-dependent kinase 2.In this study,the active substance neohesperidin was isolated from peony seed meal,which provided a theoretical basis for scientific understanding of peony seed meal waste.
作者
邹平
陈文涛
胡建刚
徐莹
张迎阳
孙承骏
夏炜芳
高蕙文
ZOU Ping;CHEN Wentao;HU Jiangang;XU Ying;ZHANG Yingyang;SUN Chengjun;XIA Weifang;GAO Huiwen(School of Biological and Food Engineering,Changzhou University,Changzhou 213164,China;Shaoxing Food and Drug Inspection Institute,Shaoxing 312071,China;Changzhou Food and Drug Fiber Quality Supervision and Inspection Center,Changzhou 213022,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2024年第9期106-116,共11页
Food and Fermentation Industries
基金
常州市科技项目(CE20212030)
浙江省市场监督管理局科技立项计划项目(20210157)
常州市科技支撑计划项目(20210157)。
关键词
新橙皮苷
大孔树脂
生物活性
分子动力
neohesperidin
macroporous resin
biological activity
molecular dynamics
作者简介
第一作者:邹平,博士,讲师;共同通信作者:邹平,讲师,E-mail:zouping@cczu.edu.com;共同通信作者:胡建刚,工程师,E-mailHujg0410@163.com。
