摘要
为获取高产纤维素酶的菌株,本研究以潮湿纤维单胞菌(Cellulomonasuda)为研究对象,采用紫外诱变方式进行菌株突变,通过刚果红选择培养基筛选,以及测定纤维素酶活性后得到菌株W3,酶活高达37.6 U/mL,较初始菌株提升了49.4%。经传代十次后仍有较高酶活,结果说明该突变菌株遗传性状稳定且产纤维素酶能力强。对突变菌株进行单因素优化实验确定最优培养条件,后对关键因子通过正交实验确定最佳参数。最佳培养条件为:12.5 g/L蔗糖,2 g/L硝酸铵,2 g/L磷酸二氢钾,菌悬液接种量体积比3%,pH=6.0,发酵培养温度35℃。优化后酶活性达43.06 U/mL,极显著高于原始菌株(P=0.008 9<0.01),较最初菌株酶活性提高了78.7%。研究结果可为高效降解纤维素提供一定理论依据和应用价值。
In order to obtain the strain with high cellulase yield,this study used Cellulomonasuda as the research objec,the strain mutation by ultraviolet mutagenesis,the selected medium of Congo red ellulase activity to obtain strain W3,with enzyme activity up to 37.6 U/mL.Compared with the initial strain,the increase was 49.4%.The high enzyme activity after ten generations,indicating that the mutant strain had stable genetic character and strong cellulase production ability.The single factor optimization experiment was to the optimal culture conditions of the mutant strain,and then the best parameters were determined by orthogonal experiment for the key factors.The optimal culture conditions were as follows:12.5 g/L sucrose,2 g/L ammonium nitrate,2 g/L potassium dihydrogen phosphate,3%inoculation volume ratio of bacterial suspension,pH=6.0,fermentation culture temperature 35℃.The optimized enzyme activity reached 43.06 U/mL,which was 78.7%higher than that of the original strain.The results can provide the oretical basis and application value for the efficient degradation of cellulose.
作者
王静蕾
李嘉琪
樊津池
宋丽芬
曲威
WANG Jinglei;LI Jiaqi;FAN Jinchi;SONG Lifen;QU Wei(Yantai Institute of China Agricultural University,Yantai,Shandong 264670,China)
出处
《陕西农业科学》
2024年第2期8-12,18,共6页
Shaanxi Journal of Agricultural Sciences
基金
烟台市校地融合发展项目(2021XDRHXMQT19)
中国农业大学烟台研究院“一流教研室”建设项目(202201Ys)。
关键词
纤维素酶
紫外诱变
发酵优化
正交实验
Cellulase
Ultraviolet mutagenesis
Fermentation optimization
Orthogonal experiment
作者简介
第一作者:王静蕾(2002-),女,在读本科,专业:设施农业;通信作者:曲威。
