摘要
为提高乳酸菌胞外多糖(EPS)产量,促进其开发及应用,本研究以植物乳杆菌(Lactiplantibacillus plantarum)HDL-03为出发菌株,通过单因素试验优化菌株产EPS的发酵条件,提高EPS产量。结果表明,L.plantarum HDL-03的最佳产EPS发酵条件为:蔗糖70 g/L、蛋白胨6 g/L、牛肉膏8 g/L、酵母浸粉7 g/L、乙酸钠1 g/L、硫酸镁0.3 g/L、磷酸氢二钾1 g/L、柠檬酸铵3 g/L、初始pH 6.5、培养温度30℃、摇床转速120 r/min、接种量3%,优化后L.plantarum HDL-03的EPS终产量为80.0 g/L,相比于优化前的EPS产量提高了2.3倍。本研究提高了乳酸菌EPS的产量,有助于其分离纯化及工业化生产。
In order to improve the yield of exopolysaccharide(EPS)of lactic acid bacteria and promote its development and application,Lactiplantibacillus plantarum HDL-03 was used as the test strain and the fermentation conditions of the EPS were optimized by single factor experiment to improve production.The results indicated that the optimal fermentation conditions of L.plantarum HDL-03 were sucrose 70 g/L,peptone 6 g/L,beef paste 8 g/L,yeast extract 7 g/L,sodium acetate 1 g/L,magnesium sulfate 0.3 g/L,dipotassium hydrogen phosphate 1 g/L,ammonium citrate 3 g/L and initial pH 6.5.Culture temperature was 30℃,shaking table rotation speed was 120 r/min and inoculum size was 3%.The final EPS yield of L.plantarum HDL-03 after optimization was 80.0 g/L,which was 2.3 times higher than the EPS yield before optimization.In this study,the yield of EPS produced by lactic acid bacteria was increased,which contributed to its separation,purification and industrial production.
作者
周渤森
曹慧莹
齐心彤
于连升
杨义
葛菁萍
宋刚
杜仁鹏
ZHOU Bosen;CAO Huiying;QI Xintong;YU Liansheng;YANG Yi;GE Jingping;SONG Gang;DU Renpeng(Engineering Research Center of Agricultural Microbiology Technology,Ministry of Education/Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region/Key Laboratory of Microbiology,College of Heilongjiang Province/School of Life Sciences,Heilongjiang University,Harbin 150080;Hebei University of Environmental Engineering/Hebei Key Laboratory of Agroecological Safety,Qinhuangdao,Hebei 066102)
出处
《中国农学通报》
2024年第11期14-21,共8页
Chinese Agricultural Science Bulletin
基金
黑龙江省自然科学基金优秀青年基金项目“乳酸明串株菌群体感应和第二信使介导右旋糖酐合成的信号通路研究”(YQ2021C030)
中国博士后科学基金资助项目“c-di-GMP和c-di-AMP介导乳酸明串珠菌L2胞外多糖合成的分子调控机制研究”(2022MD713755)
黑龙江省博士后资助项目“柠檬明串珠菌胞外多糖合成途径中信号网络的作用机制研究”(LBH-Z21082)
新时代龙江优秀硕士、博士学位论文项目“肠膜明串珠菌DRP105右旋糖酐生物合成机制的研究”(LJYXL2022-020)
河北省农业生态安全重点实验室开放基金课题项目“乳酸菌胞外多糖的分离纯化及构效关系研究”(2023SYSJJ17)
黑龙江省省属高等学校基本科研业务费科研项目“c-di-AMP调控肠膜明串珠菌DRP105胞外多糖的生物合成机理”(2022-KYYWF-1075)。
关键词
植物乳杆菌
胞外多糖
单因素
发酵条件
优化
Lactiplantibacillus plantarum
exopolysaccharide
single factor
fermentation conditions
optimization
作者简介
第一作者:周渤森,男,2000年出生,黑龙江双鸭山人,硕士研究生,研究方向:乳酸菌胞外多糖生物合成机制。通信地址:150080黑龙江哈尔滨南岗区学府路74号黑龙江大学生命科学学院,Tel:0451-86609134,E-mail:zhoubosen202304@163.com。;通信作者:宋刚,男,1979年出生,黑龙江七台河人,教授,硕士,研究方向:微生物资源挖掘与利用。通信地址:150080黑龙江哈尔滨南岗区学府路74号黑龙江大学生命科学学院,Tel:0451-86609134,E-mail:sg1150cn@126.com。;通信作者:杜仁鹏,男,1989年出生,黑龙江大兴安岭人,副教授,博士,研究方向:微生物次生代谢产物与生理。通信地址:150080黑龙江哈尔滨南岗区学府路74号黑龙江大学生命科学学院,Tel:0451-86609134,E-mail:durenpeng20096292@163.com。
