摘要
该文以苯并噻唑衍生物和二氨基马来腈为原料,经醛胺缩合制备了一种高选择性的次氯酸(HClO)荧光探针(HADM)。探针HADM在活性氧和活性氮物种的干扰下能对HClO进行快速特异性识别(反应时间<3s)且具有良好的pH稳定性,同时可以观察到明显的绿色荧光。在优化条件下,探针的荧光强度与HClO的浓度呈良好的线性关系,相关系数R~2=0.9969,检测限为2.64×10^(-6)mol/L。红外、核磁、质谱以及荧光光谱研究表明,HClO可氧化探针的HC=N键,生成了醛基化合物,发射绿色的荧光。此外,探针还成功应用于可裸眼识别的HClO试纸和复杂水样的加标回收实验。该探针制备简单、响应快、稳定性及特异性强,可作为实际应用中一种有效的次氯酸检测工具。
A highly selective hypochlorous acid fluorescent probe of HADM was prepared by aldehyde amine condensation of benzothiazole derivatives and diaminomaleonitrile.The HADM probe showed rapid and specific recognition of HClO(reaction time<3 s)with good pH stability under the interference of reactive oxygen species and reactive nitrogen species,and a clear green fluorescence could be observed.Under the optimized conditions,the fluorescence intensity of the HADM probe showed a good linear relationship with the concentration of hypochlorous acid(R 2=0.9969),and the detection limit was 2.64×10^(-6) mol/L.IR,NMR,HRMS and fluorescence spectroscopy studies showed that HC=N bond on the probe was oxidized to form an aldehyde group in the presence of HClO,resulting in generation green fluorescence.In addition,the probe was successfully applied to naked-eye identifiable HClO test strips and spiked recovery experiments of complex water samples.With the advantages of simple preparation,strong stability,good specificity,and fast response,the probe can be used as an effective tool for hypochlorous acid detection in practical applications.
作者
王丹
高云玲
WANG Dan;GAO Yunling(College of Chemical Engineering,Zhejiang University of Technology,Hangzhou 310014)
出处
《分析科学学报》
CAS
CSCD
北大核心
2024年第2期159-164,共6页
Journal of Analytical Science
关键词
次氯酸
荧光探针
苯并噻唑
Hypochlorous acid
Fluorescent probe
Benzothiazole
作者简介
通讯作者:高云玲,博士,教授,研究方向:荧光探针.E-mail:gaoyl@zjut.edu.cn。
