摘要
过氧亚硝酸盐(ONOO^(-))是机体内一种重要的活性氧物质,在众多生理和病理功能中扮演重要的角色。红色荧光探针具有背景荧光小、光毒性低和组织穿透性好等特点,在生物分析与成像方面具有明显优势。基于此,合成了一种红色ONOO^(-)荧光探针HBSCN。在与ONOO^(-)反应后,探针溶液迅速由无色变成红色,且展现出明显的荧光增强信号。通过高分辨质谱,证实该过程与探针中C=C氧化断裂有关。在PBS缓冲溶液(V(DMF)∶V(H 2O)=1∶9,pH 7.4)中,探针HBSCN在649 nm处的荧光强度与0~20μmol/L的ONOO^(-)表现出良好的线性关系,检测下限为73 nmol/L。此外,该探针能够在生理pH下快速地检测ONOO^(-),且对ONOO^(-)具有较大的Stokes位移和较高的选择性。同时HBSCN能够用于活细胞内ONOO^(-)成像,表明其潜在的生物应用价值。
Peroxynitrite(ONOO^(-))is one of important reactive oxygen species in the body,which plays a critical role in many physiological and pathological functions.Red fluorescent probes have recently attracted great interests in the fields of bioanalysis and bioimaging due to the lower background fluorescence,smaller phototoxicity and deeper tissue penetration.Based on these,we synthesized a red fluorescent ONOO^(-)fluorescent probe HBSCN.After reacting with ONOO^(-),the probe solution quickly changed from colorless to red,and showed an obvious fluorescence enhancement signal.Through high-resolution mass spectrometry,it was confirmed that the sensing process was related to the oxidative cleavage of C=C in the probe.In PBS solution(V(DMF)∶V(H 2O)=1∶9,pH 7.4),the fluorescence intensity at 649 nm of HBSCN were linearly proportional to the increasing of the concentration of ONOO^(-)(0~20μmol/L),and the low detection limit was 73 nmol/L.In addition,the probe can quickly detects ONOO^(-)under physiological pH,and displays large Stokes and high selectivity toward ONOO^(-).More importantly,HBSCN could be used for red fluorescent fluorescence imaging of ONOO^(-)in living cells,indicating its high potential value in biological application.
作者
张春香
张向阳
申有名
周紫乐
ZHANG Chun-xiang;ZHANG Xiang-yang;SHEN You-ming;ZHOU Zi-le(College of Chemistry and Materials Engineering,Hunan University of Arts and Science,Changde 415000,China)
出处
《化学试剂》
CAS
北大核心
2023年第4期1-7,共7页
Chemical Reagents
基金
湖南省自然科学基金项目(2022JJ50252)。
作者简介
张春香(1981-),女,湖南常德人,硕士,实验师,主要研究方向为有机功能材料的合成与应用,E-mail:cxzhang81@163.com。
