摘要
目的利用溶剂峰压制实验结合OPLS-DA方法分析比较不同厂家小儿止咳糖浆成分的差异,并进行有效的区分和鉴别。方法采集6个厂家21个生产批号34个样品的小儿止咳糖浆氢谱,将氢谱分段积分后,利用OPLSDA模式对数据进行分析。结果6个厂家的小儿止咳糖浆的氢谱相似,但是经过溶剂峰压制实验后发现微量成分存在差异。两次实验数据经OPLS-DA分析,结果显示不同厂家的小儿止咳糖浆成分存在差异。结论^(1)H-NMR指纹图谱结合OPLS-DA模式识别能有效区分不同厂家的小儿止咳糖浆,为小儿止咳糖浆的质量标准提供新的方法。
Objective To study the differences in the constituents of Xiao'er Zhike Tangjiang between different enterprises by solvent peaks suppression and OPLS-DA analysis,and carry out effective differentiation and identification.Methods After segmenting and integrating the hydrogen spectra of 34 samples from 21 production batches from 6 enterprises,OPLS-DA method was used to analyze the spectra.Results The H spectra of Xiao’er Zhike Tangjiang from the six enterprises were similar,however the little contents were different after solvent peaks suppression.The results of two experiments were analyzed by OPLS-DA method,which indicated that the contents were different among different manufacturers.Conclusion An effective method could distinguish Xiao'er Zhike Tangjiang from different manufacturers by ^(1)H-NMR fingerprint and OPLS-DA analysis,which provides a new method for the quality standard of Xiao'er Zhike Tangjiang.
作者
郑晓红
高荣敏
肖志会
ZHENG Xiaohong;GAO Rongmin;XIAO Zhihui(Equipment Public Service Center of South China Sea Institute of Oceanology,Guangzhou 510301,China;Minda Hospital of Hubei Minzu University,Enshi 445000,China)
出处
《广东药科大学学报》
CAS
2024年第2期63-69,共7页
Journal of Guangdong Pharmaceutical University
基金
2021年度中国科学院技术支撑人才项目(2022006)。
关键词
小儿止咳糖浆
成分差异
溶剂峰压制
OPLS-DA识别模式
Xiao’er Zhike Tangjiang
the differences of constituents
solvent peaks suppression
orthogonal partial least squares analysis
作者简介
郑晓红(1985-),女,博士,工程师,从事植物化学研究,Email:zhengxiaohong@scsio.ac.cn。
